Struct FragmentLengthDistribution 

pub struct FragmentLengthDistribution { /* private fields */ }

Tracks the observed distribution of fragment lengths.

Implementations

impl FragmentLengthDistribution

pub fn new( alpha: f64, max_val: usize, prior_mu: f64, prior_sigma: f64, kernel_n: usize, kernel_p: f64, bin_size: usize, ) -> Self

Construct a distribution.

• alpha – total pseudo-count mass (linear space).
• max_val – maximum representable length.
• prior_mu – Gaussian prior mean; if <= 0, a uniform prior is used.
• prior_sigma – Gaussian prior standard deviation.
• kernel_n – binomial kernel trials; must be even (after binning).
• kernel_p – binomial kernel success probability.
• bin_size – internal length binning (use 1 for no binning).

pub fn default_for_paired() -> Self

salmon’s default fragment-length distribution: pseudo-count 1.0, max length 1000, no Gaussian prior (uniform), kernel n=4, p=0.5.

pub fn max_val(&self) -> usize

pub fn min_val(&self) -> usize

pub fn add_val(&self, len: usize, mass: f64)

Add mass (log space) for an observed fragment of length len, spreading it over the smoothing kernel. Lock-free; safe to call from multiple threads. (Must not race with cache.)

pub fn pmf(&self, len: usize) -> f64

Logged probability of observing a fragment of length len.

pub fn refresh_online(&self)

Rebuild the online log-PMF snapshot from the current histogram (one pass over the length bins, with a single tot_mass read so the snapshot is internally consistent). Call at mini-batch boundaries during the online phase; no-op once the final cache has been taken. Cheap relative to mapping a batch, and decouples per-fragment reads from the concurrent add_val writes so identical lengths read identical values.

pub fn online_snapshot(&self) -> Arc<Vec<f64>>

Cheap (one Arc clone) immutable handle to the current online log-PMF snapshot. Capture once per fragment and index by raw length: every transcript of a given length then reads an identical value even if another thread refreshes the shared snapshot meanwhile. Empty until the first refresh_online (the pre-burn-in window, where this term is not folded into the eq-class weight anyway).

pub fn online_cmf_snapshot(&self) -> Arc<Vec<f64>>

Cheap (one Arc clone) immutable handle to the current online log-CMF snapshot, the cumulative companion to online_snapshot. Capture once per fragment for the ambiguous (orphan / single-end) fragment-length probability and the proper-pair length-conditioning. Empty until the first refresh_online.

pub fn cmf(&self, len: usize) -> f64

Logged cumulative mass up to and including len.

pub fn tot_mass(&self) -> f64

Total observed mass (log space).

pub fn mean(&self) -> f64

Mean observed length.

pub fn sd(&self) -> f64

Standard deviation of the observed length distribution, computed from the cached normalized PMF (call after cache).

pub fn cache(&mut self)

Freeze the distribution and precompute normalized PMF/CMF for fast, allocation-free lookup. Call once after updates have stopped.

pub fn log_pmf(&self) -> &[f64]

The cached, normalized log-PMF over [0, max_val]. Requires cache.

pub fn conditional_means(&self) -> Vec<f64>

Cumulative conditional means E[L | L ≤ i] over [0, max_val], i.e. salmon’s correctionFactorsFromMass (DistributionUtils.cpp): cm[i] = (Σ_{l≤i} l·pmf[l]) / (Σ_{l≤i} pmf[l]).

These are the per-length correction factors computeSmoothedEffectiveLengths subtracts from the reference length to get the base effective length. The ratio is invariant to the PMF normalization, so the cached (normalized) PMF gives the same values as salmon’s 100·exp(logPMF) mass. Requires cache.

Trait Implementations

impl Debug for FragmentLengthDistribution

fn fmt(&self, f: &mut Formatter<'_>) -> Result

Formats the value using the given formatter.