seqair 0.1.0

Pure-Rust BAM/SAM/CRAM/FASTA reader and pileup engine
Documentation
1
2
3
4
5
6
7
8
9
10
11
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
31
32
33
34
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
73
74
75
76
77
78
79
80
81
82
83
84
85
86
87
88
89
90
91
92
93
94
95
96
97
98
99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
420
421
422
423
424
425
426
427
428
429
430
431
432
433
434
435
436
437
438
439
440
441
442
443
444
445
446
447
448
449
450
451
452
453
454
455
456
457
458
459
460
461
462
463
464
465
466
467
468
469
470
471
472
473
474
475
476
477
478
479
480
481
482
483
484
485
486
487
488
489
490
491
492
493
494
495
496
497
498
499
500
501
502
503
504
505
506
507
508
509
510
511
512
513
514
515
516
517
518
519
520
521
522
523
524
525
526
527
528
529
530
531
532
533
534
535
536
537
538
539
540
541
542
543
544
545
546
547
548
549
550
551
552
553
554
555
556
557
558
559
560
561
562
563
564
565
566
567
568
569
570
571
572
573
574
575
576
577
578
579
580
581
582
583
584
585
586
587
588
589
590
591
592
593
594
595
596
597
598
599
600
601
602
603
604
605
606
607
608
609
610
611
612
613
614
615
616
617
618
619
620
621
622
623
624
625
626
627
628
629
630
631
632
633
634
635
636
637
638
639
640
641
642
643
644
645
646
647
648
649
650
651
652
653
654
655
656
657
658
659
660
661
662
663
664
665
666
667
668
669
670
671
672
673
674
675
676
677
678
679
680
681
682
683
684
685
686
687
688
689
690
691
692
693
694
695
696
697
698
699
700
701
702
703
704
705
706
707
708
709
710
711
712
713
714
715
716
717
718
719
720
721
722
723
724
725
726
727
728
729
730
731
732
733
734
735
736
737
738
739
740
741
742
743
744
745
746
747
748
749
750
751
752
753
754
755
756
757
758
759
760
761
762
763
764
765
766
767
768
769
770
771
772
773
774
775
776
777
778
779
780
781
782
783
784
785
786
787
788
789
790
791
792
793
794
795
796
797
798
799
800
//! Owned, mutable BAM record for writing and in-memory modification.
//!
//! [`OwnedBamRecord`] is the write-path entry point for the crate. Construct
//! one with [`OwnedBamRecord::builder`] (typed fields, validated at build
//! time), then hand it to [`BamWriter::write`](super::writer::BamWriter::write)
//! to serialize. [`to_bam_bytes`](OwnedBamRecord::to_bam_bytes) exposes the
//! same encoder for callers that need raw BAM bytes without going through a
//! writer.
//!
//! For the read path — bulk decode of a region's records for pileup or
//! per-read iteration — use [`RecordStore`](super::record_store::RecordStore)
//! instead. The store streams raw BAM bytes straight into slab buffers
//! without allocating per-record owned structs and is what every production
//! reader path uses. `OwnedBamRecord` exists separately because the write
//! path needs typed, mutable fields with builder ergonomics; pushing
//! pre-encoded bytes into slabs is the wrong shape for that.

use super::aux_data::{AuxData, AuxDataError};
use super::cigar::CigarOp;
use super::seq;
use crate::io::reg2bin;
use seqair_types::{BamFlags, Base, BaseQuality, Pos0};
use thiserror::Error;

/// Errors from owned record construction or serialization.
#[derive(Debug, Error)]
#[non_exhaustive]
pub enum OwnedRecordError {
    /// Query name exceeds 254 bytes (BAM `l_read_name` is u8, includes NUL).
    #[error("qname length {len} exceeds maximum of 254 bytes")]
    QnameTooLong { len: usize },

    /// CIGAR operation count exceeds 65535 (BAM `n_cigar_op` is u16).
    #[error("CIGAR op count {count} exceeds maximum of 65535")]
    CigarCountOverflow { count: usize },

    /// Sequence length exceeds `i32::MAX` (BAM `l_seq` is i32).
    #[error("sequence length {len} exceeds i32::MAX")]
    SeqLengthOverflow { len: usize },

    /// Sequence and quality score lengths do not match.
    #[error("seq length {seq_len} != qual length {qual_len}")]
    SeqQualLengthMismatch { seq_len: usize, qual_len: usize },

    /// CIGAR query-consuming length does not match sequence length.
    #[error("CIGAR query length {cigar_query_len} != seq length {seq_len}")]
    CigarSeqLengthMismatch { cigar_query_len: u64, seq_len: usize },

    /// Serialized record exceeds size limit.
    #[error("record size {size} exceeds limit")]
    RecordTooLarge { size: usize },

    /// Auxiliary tag error.
    #[error("aux data error")]
    AuxData {
        #[from]
        source: AuxDataError,
    },
}

/// Encode `Option<Pos0>` to a BAM-wire `i32`. `None` → `-1`.
#[inline]
fn pos_to_bam_i32(p: Option<Pos0>) -> i32 {
    p.map_or(-1, Pos0::as_i32)
}

// r[impl bam.owned_record.fields]
/// An owned, mutable BAM record for writing and in-memory modification.
#[derive(Debug, Clone)]
pub struct OwnedBamRecord {
    /// Reference sequence index. `-1` is the canonical "unmapped" sentinel
    /// per [SAM1] §1.4 — it is preserved here as a raw `i32` (not wrapped in
    /// an `Option`) because reading code uses it as a header-table index and
    /// every check site already handles the -1 case explicitly.
    pub ref_id: i32,
    /// 0-based leftmost position. `None` for unmapped/unavailable (BAM
    /// wire `-1`); `Some(p)` for any in-range mapping. Construction via
    /// [`Pos0`] makes overflow unconstructable.
    pub pos: Option<Pos0>,
    pub mapq: u8,
    pub flags: BamFlags,
    /// Mate's reference sequence index. `-1` for unavailable (same rationale
    /// as `ref_id`).
    pub next_ref_id: i32,
    /// Mate's 0-based position. `None` for unavailable (BAM wire `-1`).
    pub next_pos: Option<Pos0>,
    pub template_len: i32,
    pub qname: Vec<u8>,
    pub cigar: Vec<CigarOp>,
    pub seq: Vec<Base>,
    // r[impl types.base_quality.field_type]
    pub qual: Vec<BaseQuality>,
    pub aux: AuxData,
}

/// Builder for constructing [`OwnedBamRecord`] values.
pub struct OwnedBamRecordBuilder {
    ref_id: i32,
    pos: Option<Pos0>,
    mapq: u8,
    flags: BamFlags,
    next_ref_id: i32,
    next_pos: Option<Pos0>,
    template_len: i32,
    qname: Vec<u8>,
    cigar: Vec<CigarOp>,
    seq: Vec<Base>,
    qual: Vec<BaseQuality>,
    aux: AuxData,
}

// r[impl bam.owned_record.builder]
impl OwnedBamRecordBuilder {
    pub fn flags(mut self, flags: BamFlags) -> Self {
        self.flags = flags;
        self
    }
    pub fn mapq(mut self, mapq: u8) -> Self {
        self.mapq = mapq;
        self
    }
    pub fn cigar(mut self, cigar: Vec<CigarOp>) -> Self {
        self.cigar = cigar;
        self
    }
    pub fn seq(mut self, seq: Vec<Base>) -> Self {
        self.seq = seq;
        self
    }
    pub fn qual(mut self, qual: Vec<BaseQuality>) -> Self {
        self.qual = qual;
        self
    }
    pub fn next_ref_id(mut self, v: i32) -> Self {
        self.next_ref_id = v;
        self
    }
    pub fn next_pos(mut self, v: Option<Pos0>) -> Self {
        self.next_pos = v;
        self
    }
    pub fn template_len(mut self, v: i32) -> Self {
        self.template_len = v;
        self
    }
    pub fn aux(mut self, aux: AuxData) -> Self {
        self.aux = aux;
        self
    }

    // r[impl bam.owned_record.qname_limit]
    // r[impl bam.owned_record.cigar_count_limit]
    // r[impl bam.owned_record.seq_length_limit]
    pub fn build(self) -> Result<OwnedBamRecord, OwnedRecordError> {
        if self.qname.len() > 254 {
            return Err(OwnedRecordError::QnameTooLong { len: self.qname.len() });
        }
        if self.cigar.len() > 65535 {
            return Err(OwnedRecordError::CigarCountOverflow { count: self.cigar.len() });
        }
        if self.seq.len() > i32::MAX as usize {
            return Err(OwnedRecordError::SeqLengthOverflow { len: self.seq.len() });
        }
        if !self.qual.is_empty() && self.qual.len() != self.seq.len() {
            return Err(OwnedRecordError::SeqQualLengthMismatch {
                seq_len: self.seq.len(),
                qual_len: self.qual.len(),
            });
        }

        Ok(OwnedBamRecord {
            ref_id: self.ref_id,
            pos: self.pos,
            mapq: self.mapq,
            flags: self.flags,
            next_ref_id: self.next_ref_id,
            next_pos: self.next_pos,
            template_len: self.template_len,
            qname: self.qname,
            cigar: self.cigar,
            seq: self.seq,
            qual: self.qual,
            aux: self.aux,
        })
    }
}

impl OwnedBamRecord {
    /// Start building a record with the required fields.
    ///
    /// Pass `None` for `pos` to mark the record as unmapped (BAM wire `-1`).
    pub fn builder(ref_id: i32, pos: Option<Pos0>, qname: Vec<u8>) -> OwnedBamRecordBuilder {
        OwnedBamRecordBuilder {
            ref_id,
            pos,
            mapq: 0,
            flags: BamFlags::empty(),
            next_ref_id: -1,
            next_pos: None,
            template_len: 0,
            qname,
            cigar: Vec::new(),
            seq: Vec::new(),
            qual: Vec::new(),
            aux: AuxData::new(),
        }
    }

    // r[impl bam.owned_record.flag_methods]
    pub fn set_flags(&mut self, flags: BamFlags) {
        self.flags = flags;
    }

    pub fn is_unmapped(&self) -> bool {
        self.flags.is_unmapped()
    }

    pub fn is_reverse(&self) -> bool {
        self.flags.is_reverse()
    }

    // r[impl bam.owned_record.end_pos]
    /// Compute the 0-based exclusive end position from pos + reference-consuming CIGAR ops.
    /// Returns `None` if the record is unmapped (`pos.is_none()`).
    pub fn end_pos(&self) -> Option<Pos0> {
        let pos = self.pos?;
        let ref_len: u64 =
            self.cigar.iter().filter(|op| op.consumes_ref()).map(|op| u64::from(op.len())).sum();
        let end = pos.as_u64().saturating_add(ref_len);
        let clamped = end.min(Pos0::max_value().as_u64());
        #[expect(
            clippy::cast_possible_truncation,
            reason = "clamped <= i32::MAX < u32::MAX; fits in u32"
        )]
        Pos0::new(clamped as u32)
    }

    // r[impl bam.owned_record.bin]
    /// Compute the BAI bin value from pos and `end_pos` (BAI scheme: `min_shift=14`, depth=5).
    /// For unmapped records (`pos.is_none()`), returns the unmapped bin (`reg2bin(0, 1, ...)`).
    pub fn bin(&self) -> u16 {
        let beg = self.pos.map(Pos0::as_u64).unwrap_or(0);
        let end = self.end_pos().map_or(beg.saturating_add(1), |p| {
            let v = p.as_u64();
            if v <= beg { beg.saturating_add(1) } else { v }
        });
        #[expect(
            clippy::cast_possible_truncation,
            reason = "BAI bin numbers with min_shift=14, depth=5 are bounded by 37449, fits in u16"
        )]
        let bin = reg2bin(beg, end, 14, 5) as u16;
        bin
    }

    /// Query-consuming length of the CIGAR.
    pub fn cigar_query_len(&self) -> u64 {
        self.cigar.iter().filter(|op| op.consumes_query()).map(|op| u64::from(op.len())).sum()
    }

    // r[impl bam.owned_record.set_alignment]
    /// Replace alignment (pos + cigar). Validates CIGAR query length == seq length for mapped reads.
    pub fn set_alignment(
        &mut self,
        pos: Option<Pos0>,
        cigar: Vec<CigarOp>,
    ) -> Result<(), OwnedRecordError> {
        if cigar.len() > 65535 {
            return Err(OwnedRecordError::CigarCountOverflow { count: cigar.len() });
        }
        // For mapped reads, validate CIGAR query length matches seq length
        if !self.is_unmapped() && !self.seq.is_empty() {
            let query_len: u64 =
                cigar.iter().filter(|op| op.consumes_query()).map(|op| u64::from(op.len())).sum();
            if query_len != self.seq.len() as u64 {
                return Err(OwnedRecordError::CigarSeqLengthMismatch {
                    cigar_query_len: query_len,
                    seq_len: self.seq.len(),
                });
            }
        }
        self.pos = pos;
        self.cigar = cigar;
        Ok(())
    }

    // r[impl bam.owned_record.set_seq]
    /// Replace the sequence. For mapped reads, validates length matches CIGAR query length.
    pub fn set_seq(&mut self, seq: Vec<Base>) -> Result<(), OwnedRecordError> {
        if !self.is_unmapped() && !self.cigar.is_empty() {
            let query_len = self.cigar_query_len();
            if query_len != seq.len() as u64 {
                return Err(OwnedRecordError::CigarSeqLengthMismatch {
                    cigar_query_len: query_len,
                    seq_len: seq.len(),
                });
            }
        }
        self.seq = seq;
        Ok(())
    }

    /// Mutable access to the sequence for in-place base modification.
    pub fn seq_mut(&mut self) -> &mut [Base] {
        &mut self.seq
    }

    // r[impl bam.owned_record.set_qual]
    /// Replace quality scores. Length must equal seq length.
    pub fn set_qual(&mut self, qual: Vec<BaseQuality>) -> Result<(), OwnedRecordError> {
        if !qual.is_empty() && qual.len() != self.seq.len() {
            return Err(OwnedRecordError::SeqQualLengthMismatch {
                seq_len: self.seq.len(),
                qual_len: qual.len(),
            });
        }
        self.qual = qual;
        Ok(())
    }

    // r[impl bam.owned_record.to_bam_bytes]
    // r[impl bam.owned_record.to_bam_bin_field]
    /// Serialize to BAM binary format by appending into `buf`.
    ///
    /// Does NOT include the 4-byte `block_size` prefix — that is the writer's responsibility.
    pub fn to_bam_bytes(&self, buf: &mut Vec<u8>) -> Result<(), OwnedRecordError> {
        // Validate field limits. `pos`/`next_pos` are constructively bounded
        // by `Option<Pos0>` (Pos0 ≤ i32::MAX) so no overflow check is needed.
        if self.qname.len() > 254 {
            return Err(OwnedRecordError::QnameTooLong { len: self.qname.len() });
        }
        if self.cigar.len() > 65535 {
            return Err(OwnedRecordError::CigarCountOverflow { count: self.cigar.len() });
        }
        if self.seq.len() > i32::MAX as usize {
            return Err(OwnedRecordError::SeqLengthOverflow { len: self.seq.len() });
        }

        #[expect(
            clippy::cast_possible_truncation,
            reason = "qname.len() ≤ 254 (validated above); +1 fits in u8"
        )]
        let l_read_name = (self.qname.len() as u8).saturating_add(1); // +1 for NUL
        #[expect(
            clippy::cast_possible_truncation,
            reason = "cigar.len() ≤ 65535 (validated above); fits in u16"
        )]
        let n_cigar_op = self.cigar.len() as u16;
        #[expect(
            clippy::cast_possible_truncation,
            clippy::cast_possible_wrap,
            reason = "seq.len() ≤ i32::MAX (validated above); fits in i32"
        )]
        let l_seq = self.seq.len() as i32;

        super::record::encode_fixed_header(
            buf,
            &super::record::FixedHeaderFields {
                ref_id: self.ref_id,
                pos: pos_to_bam_i32(self.pos),
                bin: self.bin(),
                mapq: self.mapq,
                l_read_name,
                flags: self.flags.raw(),
                n_cigar_op,
                l_seq,
                next_ref_id: self.next_ref_id,
                next_pos: pos_to_bam_i32(self.next_pos),
                template_len: self.template_len,
            },
        );

        // qname + NUL
        buf.extend_from_slice(&self.qname);
        buf.push(0);

        // CIGAR: each op as u32 LE
        for op in &self.cigar {
            buf.extend_from_slice(&op.to_bam_u32().to_le_bytes());
        }

        // Sequence: encode Base values to 4-bit packed
        if !self.seq.is_empty() {
            // Base is #[repr(u8)] with ASCII discriminants (A=0x41, C=0x43, G=0x47, T=0x54, N=0x4E).
            // The compile-time assert in seqair-types guarantees size_of::<Base>() == 1.
            // We collect to a byte vec rather than using unsafe transmute.
            let seq_bytes: Vec<u8> = self.seq.iter().map(|b| *b as u8).collect();
            let encoded = seq::encode_seq(&seq_bytes);
            buf.extend_from_slice(&encoded);
        }

        // Quality scores
        if self.qual.is_empty() {
            // Missing qual: fill with 0xFF per SAM spec
            buf.resize(buf.len().saturating_add(self.seq.len()), 0xFF);
        } else {
            buf.extend_from_slice(BaseQuality::slice_to_bytes(&self.qual));
        }

        // Auxiliary data
        buf.extend_from_slice(self.aux.as_bytes());

        Ok(())
    }
}

#[cfg(test)]
#[allow(clippy::arithmetic_side_effects, reason = "test arithmetic on known small values")]
mod tests {
    use super::super::cigar::CigarOpType;
    use super::*;
    use seqair_types::Pos0;

    fn simple_record() -> OwnedBamRecord {
        OwnedBamRecord::builder(0, Some(Pos0::new(100).unwrap()), b"read1".to_vec())
            .flags(BamFlags::empty()) // mapped
            .mapq(30)
            .cigar(vec![CigarOp::new(CigarOpType::Match, 5)])
            .seq(vec![Base::A, Base::C, Base::G, Base::T, Base::A])
            .qual([30, 31, 32, 33, 34].map(BaseQuality::from_byte).to_vec())
            .build()
            .unwrap()
    }

    use super::super::test_util::decode_into_store;

    // r[verify bam.owned_record.to_bam_bytes]
    // r[verify bam.owned_record.test_roundtrip_bytes]
    #[test]
    fn serialize_and_decode_roundtrip() {
        let rec = simple_record();
        let mut buf = Vec::new();
        rec.to_bam_bytes(&mut buf).unwrap();

        let store = decode_into_store(&buf);
        let decoded = store.record(0);

        assert_eq!(decoded.tid, 0);
        assert_eq!(*decoded.pos, 100);
        assert_eq!(decoded.mapq, 30);
        assert_eq!(decoded.flags, BamFlags::empty());
        assert_eq!(decoded.seq_len, 5);
        assert_eq!(decoded.n_cigar_ops, 1);
        assert_eq!(store.qname(0), b"read1");
    }

    #[test]
    fn serialize_with_aux_tags() {
        let mut aux = AuxData::new();
        aux.set_int(*b"NM", 3).unwrap();
        aux.set_string(*b"RG", b"grp1");

        let rec = OwnedBamRecord::builder(0, Some(Pos0::new(100).unwrap()), b"r1".to_vec())
            .cigar(vec![CigarOp::new(CigarOpType::Match, 3)])
            .seq(vec![Base::A, Base::C, Base::G])
            .qual([30, 31, 32].map(BaseQuality::from_byte).to_vec())
            .aux(aux)
            .build()
            .unwrap();

        let mut buf = Vec::new();
        rec.to_bam_bytes(&mut buf).unwrap();

        let store = decode_into_store(&buf);
        let aux = store.aux(0);
        let nm = super::super::aux::find_tag(aux, *b"NM");
        assert_eq!(nm, Some(super::super::aux::AuxValue::U8(3)));

        let rg = super::super::aux::find_tag(aux, *b"RG");
        assert_eq!(rg, Some(super::super::aux::AuxValue::String(b"grp1")));
    }

    // r[verify bam.owned_record.to_bam_bin_field]
    #[test]
    fn bin_recomputed_after_modification() {
        let mut rec = simple_record(); // pos=100, 5M
        let bin_before = rec.bin();

        // Move to a completely different position — bin must change
        rec.set_alignment(
            Some(Pos0::new(10_000_000).unwrap()),
            vec![CigarOp::new(CigarOpType::Match, 5)],
        )
        .unwrap();
        let bin_after = rec.bin();
        assert_ne!(bin_before, bin_after, "bin must change when pos changes");

        // Serialize and verify the bin in the bytes matches the new value
        let mut buf = Vec::new();
        rec.to_bam_bytes(&mut buf).unwrap();
        // bin_mq_nl is at offset 8 in the 32-byte header (bytes 8-11)
        let bin_mq_nl = u32::from_le_bytes([buf[8], buf[9], buf[10], buf[11]]);
        let serialized_bin = (bin_mq_nl >> 16) as u16;
        assert_eq!(serialized_bin, bin_after, "serialized bin must match current bin()");
    }

    // r[verify bam.owned_record.end_pos]
    #[test]
    fn end_pos_simple() {
        let rec = simple_record();
        assert_eq!(rec.end_pos(), Some(Pos0::new(105).unwrap())); // pos=100 + 5M
    }

    #[test]
    fn end_pos_with_insertion() {
        let rec = OwnedBamRecord::builder(0, Some(Pos0::new(100).unwrap()), b"r".to_vec())
            .cigar(vec![
                CigarOp::new(CigarOpType::Match, 3),
                CigarOp::new(CigarOpType::Insertion, 2),
                CigarOp::new(CigarOpType::Match, 3),
            ])
            .seq(vec![Base::A; 8]) // 3M + 2I + 3M = 8 query bases
            .qual(vec![BaseQuality::from_byte(30); 8])
            .build()
            .unwrap();
        // Only M ops consume ref: 3 + 3 = 6
        assert_eq!(rec.end_pos(), Some(Pos0::new(106).unwrap()));
    }

    // r[verify bam.owned_record.qname_limit]
    #[test]
    fn qname_too_long_rejected() {
        let long_name = vec![b'A'; 255];
        let result = OwnedBamRecord::builder(0, None, long_name).build();
        assert!(result.is_err());
    }

    #[test]
    fn qname_at_limit_accepted() {
        let name = vec![b'A'; 254];
        let result = OwnedBamRecord::builder(0, None, name).build();
        assert!(result.is_ok());
    }

    // r[verify bam.owned_record.set_alignment]
    // r[verify bam.owned_record.test_modification]
    #[test]
    fn set_alignment_validates_cigar_seq_mismatch() {
        let mut rec = simple_record(); // seq len = 5
        let result = rec.set_alignment(
            Some(Pos0::new(200).unwrap()),
            vec![CigarOp::new(CigarOpType::Match, 10)], // query len = 10 != 5
        );
        assert!(result.is_err());
    }

    #[test]
    fn set_alignment_succeeds_when_matching() {
        let mut rec = simple_record(); // seq len = 5
        let result = rec.set_alignment(
            Some(Pos0::new(200).unwrap()),
            vec![CigarOp::new(CigarOpType::Match, 5)],
        );
        assert!(result.is_ok());
        assert_eq!(rec.pos, Some(Pos0::new(200).unwrap()));
        assert_eq!(rec.end_pos(), Some(Pos0::new(205).unwrap()));
    }

    #[test]
    fn empty_qual_fills_with_0xff() {
        let rec = OwnedBamRecord::builder(0, Some(Pos0::new(100).unwrap()), b"r".to_vec())
            .cigar(vec![CigarOp::new(CigarOpType::Match, 3)])
            .seq(vec![Base::A, Base::C, Base::G])
            // no qual set
            .build()
            .unwrap();

        let mut buf = Vec::new();
        rec.to_bam_bytes(&mut buf).unwrap();

        let store = decode_into_store(&buf);
        assert!(store.qual(0).iter().all(|&q| q == BaseQuality::UNAVAILABLE));
    }

    #[test]
    fn unmapped_record_serializes() {
        // Use ref_id=-1 and pos=None for a fully unmapped record.
        // The existing reader rejects pos=-1 (InvalidPosition), so we verify
        // serialization succeeds and the bytes have the right structure.
        let rec = OwnedBamRecord::builder(-1, None, b"unmapped".to_vec())
            .flags(BamFlags::from(0x4))
            .seq(vec![Base::A, Base::C, Base::G])
            .build()
            .unwrap();

        let mut buf = Vec::new();
        rec.to_bam_bytes(&mut buf).unwrap();

        // Verify the fixed header bytes directly
        let ref_id = i32::from_le_bytes([buf[0], buf[1], buf[2], buf[3]]);
        let pos = i32::from_le_bytes([buf[4], buf[5], buf[6], buf[7]]);
        assert_eq!(ref_id, -1);
        assert_eq!(pos, -1);
    }

    #[test]
    fn placed_unmapped_record_roundtrips() {
        // Placed unmapped: has ref_id and pos, but flag 0x4 set
        let rec = OwnedBamRecord::builder(0, Some(Pos0::new(500).unwrap()), b"placed".to_vec())
            .flags(BamFlags::from(0x4))
            .seq(vec![Base::A, Base::C, Base::G])
            .build()
            .unwrap();

        let mut buf = Vec::new();
        rec.to_bam_bytes(&mut buf).unwrap();

        let store = decode_into_store(&buf);
        let decoded = store.record(0);
        assert_eq!(decoded.n_cigar_ops, 0);
        assert_eq!(decoded.seq_len, 3);
        assert_eq!(decoded.flags & BamFlags::from(0x4), BamFlags::from(0x4));
    }

    // r[verify cigar.aligned_pairs.owned_record]
    #[test]
    fn aligned_pairs_simple_match() {
        use super::super::aligned_pairs::{AlignedPair, MatchKind};
        let rec = simple_record(); // 5M at pos 100
        let pairs: Vec<_> = rec.aligned_pairs().unwrap().collect();
        assert_eq!(pairs.len(), 5);
        assert_eq!(
            pairs[0],
            AlignedPair::Match { qpos: 0, rpos: Pos0::new(100).unwrap(), kind: MatchKind::Match }
        );
        assert_eq!(
            pairs[4],
            AlignedPair::Match { qpos: 4, rpos: Pos0::new(104).unwrap(), kind: MatchKind::Match }
        );
    }

    #[test]
    fn aligned_pairs_with_insertion() {
        use super::super::aligned_pairs::{AlignedPair, MatchKind};
        let rec = OwnedBamRecord::builder(0, Some(Pos0::new(100).unwrap()), b"r".to_vec())
            .cigar(vec![
                CigarOp::new(CigarOpType::Match, 2),
                CigarOp::new(CigarOpType::Insertion, 1),
                CigarOp::new(CigarOpType::Match, 2),
            ])
            .seq(vec![Base::A; 5])
            .qual(vec![BaseQuality::from_byte(30); 5])
            .build()
            .unwrap();

        let pairs: Vec<_> = rec.aligned_pairs().unwrap().collect();
        let m = |q, r| AlignedPair::Match {
            qpos: q,
            rpos: Pos0::new(r).unwrap(),
            kind: MatchKind::Match,
        };
        assert_eq!(pairs.len(), 5);
        assert_eq!(pairs[0], m(0, 100));
        assert_eq!(pairs[1], m(1, 101));
        assert_eq!(pairs[2], AlignedPair::Insertion { qpos: 2, insert_len: 1 });
        assert_eq!(pairs[3], m(3, 102));
        assert_eq!(pairs[4], m(4, 103));
    }

    #[test]
    fn aligned_pairs_with_deletion() {
        use super::super::aligned_pairs::{AlignedPair, MatchKind};
        let rec = OwnedBamRecord::builder(0, Some(Pos0::new(100).unwrap()), b"r".to_vec())
            .cigar(vec![
                CigarOp::new(CigarOpType::Match, 2),
                CigarOp::new(CigarOpType::Deletion, 3),
                CigarOp::new(CigarOpType::Match, 2),
            ])
            .seq(vec![Base::A; 4])
            .qual(vec![BaseQuality::from_byte(30); 4])
            .build()
            .unwrap();

        let pairs: Vec<_> = rec.aligned_pairs().unwrap().collect();
        let m = |q, r| AlignedPair::Match {
            qpos: q,
            rpos: Pos0::new(r).unwrap(),
            kind: MatchKind::Match,
        };
        assert_eq!(pairs.len(), 5); // 2M + D(summary) + 2M = 5
        assert_eq!(pairs[0], m(0, 100));
        assert_eq!(pairs[1], m(1, 101));
        // Deletion summary: rpos=102, del_len=3
        assert_eq!(pairs[2], AlignedPair::Deletion { rpos: Pos0::new(102).unwrap(), del_len: 3 });
        assert_eq!(pairs[3], m(2, 105));
        assert_eq!(pairs[4], m(3, 106));
    }

    #[test]
    fn aligned_pairs_with_soft_clip() {
        use super::super::aligned_pairs::{AlignedPair, MatchKind};
        let rec = OwnedBamRecord::builder(0, Some(Pos0::new(100).unwrap()), b"r".to_vec())
            .cigar(vec![
                CigarOp::new(CigarOpType::SoftClip, 2),
                CigarOp::new(CigarOpType::Match, 3),
            ])
            .seq(vec![Base::A; 5])
            .qual(vec![BaseQuality::from_byte(30); 5])
            .build()
            .unwrap();

        let pairs: Vec<_> = rec.aligned_pairs().unwrap().collect();
        let m = |q, r| AlignedPair::Match {
            qpos: q,
            rpos: Pos0::new(r).unwrap(),
            kind: MatchKind::Match,
        };
        // Soft clips are skipped by default, only 3 Match pairs
        assert_eq!(pairs.len(), 3);
        assert_eq!(pairs[0], m(2, 100));
        assert_eq!(pairs[2], m(4, 102));
    }

    #[test]
    fn aligned_pairs_empty_cigar() {
        let rec = OwnedBamRecord::builder(-1, None, b"r".to_vec())
            .flags(BamFlags::from(0x4))
            .build()
            .unwrap();
        let pairs: Vec<_> = rec.aligned_pairs().unwrap().collect();
        assert!(pairs.is_empty());
    }

    // r[verify cigar.aligned_pairs.owned_record]
    #[test]
    fn aligned_pairs_rejects_unmapped_with_cigar() {
        // Degenerate but constructible: pos = None + non-empty CIGAR. Walking
        // would produce nonsense rpos values anchored at Pos0::ZERO, so we
        // refuse rather than silently corrupt the output.
        let rec = OwnedBamRecord::builder(-1, None, b"r".to_vec())
            .flags(BamFlags::from(0x4))
            .cigar(vec![CigarOp::new(CigarOpType::Match, 5)])
            .seq(vec![Base::A; 5])
            .qual(vec![BaseQuality::from_byte(30); 5])
            .build()
            .unwrap();
        let result = rec.aligned_pairs();
        assert!(matches!(
            result,
            Err(super::super::aligned_pairs::AlignedPairsError::UnmappedWithCigar { cigar_ops: 1 })
        ));
    }

    // r[verify cigar.aligned_pairs.with_read.owned_record]
    #[test]
    fn aligned_pairs_with_read_attaches_query_base() {
        // 3M record with seq [A, C, G] — verify the helper attaches per-pos
        // bases without going through the SlimRecord/RecordStore round-trip.
        use super::super::aligned_pairs_view::AlignedPairWithRead;
        let rec = OwnedBamRecord::builder(0, Some(Pos0::new(50).unwrap()), b"r".to_vec())
            .flags(BamFlags::empty())
            .cigar(vec![CigarOp::new(CigarOpType::Match, 3)])
            .seq(vec![Base::A, Base::C, Base::G])
            .qual([30, 31, 32].map(BaseQuality::from_byte).to_vec())
            .build()
            .unwrap();

        let events: Vec<_> = rec.aligned_pairs_with_read().unwrap().collect();
        assert_eq!(events.len(), 3);
        match events[0] {
            AlignedPairWithRead::Match { qpos: 0, query: Base::A, .. } => {}
            other => panic!("expected Match{{qpos=0, query=A}}, got {other:?}"),
        }
        match events[2] {
            AlignedPairWithRead::Match { qpos: 2, query: Base::G, .. } => {}
            other => panic!("expected Match{{qpos=2, query=G}}, got {other:?}"),
        }
    }

    // r[verify cigar.aligned_pairs.with_read.owned_record]
    #[test]
    fn aligned_pairs_with_read_propagates_unmapped_error() {
        // pos = None + non-empty CIGAR → UnmappedWithCigar surfaces through
        // the helper just like through the bare aligned_pairs().
        let rec = OwnedBamRecord::builder(-1, None, b"r".to_vec())
            .flags(BamFlags::from(0x4))
            .cigar(vec![CigarOp::new(CigarOpType::Match, 3)])
            .seq(vec![Base::A; 3])
            .qual(vec![BaseQuality::from_byte(30); 3])
            .build()
            .unwrap();
        let result = rec.aligned_pairs_with_read();
        assert!(matches!(
            result,
            Err(super::super::aligned_pairs::AlignedPairsError::UnmappedWithCigar { cigar_ops: 1 })
        ));
    }

    // r[verify cigar.aligned_pairs.owned_record]
    #[test]
    fn aligned_pairs_accepts_unmapped_with_empty_cigar() {
        // The "fully unmapped" case: pos = None AND cigar = empty. Iteration
        // yields nothing — no rpos anchoring needed.
        let rec = OwnedBamRecord::builder(-1, None, b"r".to_vec())
            .flags(BamFlags::from(0x4))
            .build()
            .unwrap();
        let pairs: Vec<_> = rec.aligned_pairs().unwrap().collect();
        assert!(pairs.is_empty());
    }
}