use std::{
fs::File,
io::{self, BufRead, BufReader, Read},
path::Path,
};
use noodles_bam as bam;
use noodles_bgzf as bgzf;
use noodles_cram as cram;
use noodles_fasta as fasta;
use noodles_sam as sam;
use super::Reader;
use crate::alignment::Format;
#[derive(Default)]
pub struct Builder {
format: Option<Format>,
reference_sequence_repository: fasta::Repository,
}
impl Builder {
pub fn set_format(mut self, format: Format) -> Self {
self.format = Some(format);
self
}
pub fn set_reference_sequence_repository(
mut self,
reference_sequence_repository: fasta::Repository,
) -> Self {
self.reference_sequence_repository = reference_sequence_repository;
self
}
pub fn build_from_path<P>(self, path: P) -> io::Result<Reader<Box<dyn BufRead>>>
where
P: AsRef<Path>,
{
let file = File::open(path)?;
self.build_from_reader(file)
}
pub fn build_from_reader<R>(self, reader: R) -> io::Result<Reader<Box<dyn BufRead>>>
where
R: Read + 'static,
{
let mut reader = BufReader::new(reader);
let format = self
.format
.map(Ok)
.unwrap_or_else(|| detect_format(&mut reader))?;
let inner: Box<dyn sam::AlignmentReader<_>> = match format {
Format::Sam => {
let inner: Box<dyn BufRead> = Box::new(reader);
Box::new(sam::Reader::from(inner))
}
Format::SamGz => {
let inner: Box<dyn BufRead> = Box::new(bgzf::Reader::new(reader));
Box::new(sam::Reader::from(inner))
}
Format::Bam => {
let inner: Box<dyn BufRead> = Box::new(bgzf::Reader::new(reader));
Box::new(bam::Reader::from(inner))
}
Format::Cram => {
let inner: Box<dyn BufRead> = Box::new(reader);
Box::new(
cram::reader::Builder::default()
.set_reference_sequence_repository(self.reference_sequence_repository)
.build_from_reader(inner),
)
}
};
Ok(Reader { inner })
}
}
fn detect_format<R>(reader: &mut R) -> io::Result<Format>
where
R: BufRead,
{
const CRAM_MAGIC_NUMBER: [u8; 4] = [b'C', b'R', b'A', b'M'];
const GZIP_MAGIC_NUMBER: [u8; 2] = [0x1f, 0x8b];
const BAM_MAGIC_NUMBER: [u8; 4] = [b'B', b'A', b'M', 0x01];
let src = reader.fill_buf()?;
if let Some(buf) = src.get(..4) {
if buf == CRAM_MAGIC_NUMBER {
return Ok(Format::Cram);
}
if buf[..2] == GZIP_MAGIC_NUMBER {
let mut reader = bgzf::Reader::new(src);
let mut buf = [0; 4];
reader.read_exact(&mut buf).ok();
if buf == BAM_MAGIC_NUMBER {
return Ok(Format::Bam);
} else {
return Ok(Format::SamGz);
}
}
}
Ok(Format::Sam)
}
#[cfg(test)]
mod tests {
use super::*;
#[test]
fn test_detect_format() -> io::Result<()> {
use std::io::Write;
fn t(mut src: &[u8], expected: Format) {
assert!(matches!(detect_format(&mut src), Ok(value) if value == expected));
}
t(b"@HD\tVN:1.6\n", Format::Sam);
t(b"", Format::Sam);
let mut writer = bgzf::Writer::new(Vec::new());
writer.write_all(b"@HD\tVN:1.6\n")?;
let src = writer.finish()?;
t(&src, Format::SamGz);
let mut writer = bgzf::Writer::new(Vec::new());
writer.write_all(b"BAM\x01")?;
let src = writer.finish()?;
t(&src, Format::Bam);
t(b"CRAM", Format::Cram);
Ok(())
}
}