1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 34 35 36 37 38 39 40 41 42 43 44 45 46 47 48 49 50 51 52 53 54 55 56 57 58 59 60 61 62 63 64 65 66 67 68 69 70 71 72 73 74 75 76 77 78 79 80 81 82 83 84 85 86 87 88 89 90 91 92 93 94 95 96 97 98 99 100 101 102 103 104 105 106 107 108 109 110 111 112 113 114 115 116 117 118 119 120 121 122 123 124 125 126 127 128 129 130 131 132 133 134 135 136 137 138 139 140 141 142 143 144 145 146 147 148 149 150 151 152 153 154 155 156 157 158 159 160 161 162 163 164 165 166 167 168 169 170 171 172 173 174 175 176 177 178 179 180 181 182 183 184 185 186 187 188 189 190 191 192 193 194 195 196 197 198 199 200 201 202 203 204 205 206 207 208 209 210 211 212 213 214 215 216 217 218 219 220 221 222 223 224 225 226 227 228 229 230 231 232 233 234 235 236 237 238 239 240 241 242 243 244 245 246 247 248 249 250 251 252 253 254 255 256 257 258 259 260 261 262 263 264 265 266 267 268 269 270 271 272 273 274 275 276 277 278 279 280 281 282 283 284 285 286 287 288 289 290 291 292 293 294 295 296 297 298 299 300 301 302 303 304 305 306 307 308 309 310 311 312 313 314 315 316 317 318 319 320 321 322 323 324 325 326 327 328 329 330 331 332 333 334 335 336 337 338 339 340 341 342 343 344 345 346 347 348 349 350 351 352 353 354 355 356 357 358 359 360 361 362 363 364 365 366 367 368 369 370 371 372 373 374 375 376 377 378 379 380 381 382 383 384 385 386 387 388 389 390 391 392 393 394 395 396 397 398 399 400 401 402 403 404 405 406 407 408 409 410 411 412 413 414 415 416 417 418 419 420 421 422 423 424 425 426 427 428 429 430 431 432 433 434 435 436 437 438 439 440 441 442 443
mod lazy_record;
mod query;
use futures::{stream, Stream};
use noodles_bgzf as bgzf;
use noodles_core::Region;
use noodles_csi as csi;
use tokio::io::{self, AsyncRead, AsyncReadExt, AsyncSeek};
use self::{lazy_record::read_lazy_record, query::query};
use crate::{header::string_maps::ContigStringMap, lazy};
/// An async BCF reader.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use futures::TryStreamExt;
/// use noodles_bcf as bcf;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bcf").await.map(bcf::AsyncReader::new)?;
/// reader.read_file_format().await?;
/// reader.read_header().await?;
///
/// let mut records = reader.lazy_records();
///
/// while let Some(record) = records.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub struct Reader<R> {
inner: R,
buf: Vec<u8>,
}
impl<R> Reader<R>
where
R: AsyncRead + Unpin,
{
/// Returns a reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_bcf as bcf;
/// let data = [];
/// let reader = bcf::AsyncReader::from(&data[..]);
/// assert!(reader.get_ref().is_empty());
/// ```
pub fn get_ref(&self) -> &R {
&self.inner
}
/// Returns a mutable reference to the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_bcf as bcf;
/// let data = [];
/// let mut reader = bcf::AsyncReader::from(&data[..]);
/// assert!(reader.get_mut().is_empty());
/// ```
pub fn get_mut(&mut self) -> &mut R {
&mut self.inner
}
/// Returns the underlying reader.
///
/// # Examples
///
/// ```
/// use noodles_bcf as bcf;
/// let data = [];
/// let reader = bcf::AsyncReader::from(&data[..]);
/// assert!(reader.into_inner().is_empty());
/// ```
pub fn into_inner(self) -> R {
self.inner
}
/// Reads the BCF file format.
///
/// The BCF magic number is also checked.
///
/// The position of the stream is expected to be at the start.
///
/// This returns the major and minor format versions as a tuple.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bcf as bcf;
/// use tokio::fs::File;
/// let mut reader = File::open("sample.bcf").await.map(bcf::AsyncReader::new)?;
/// let (major, minor) = reader.read_file_format().await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_file_format(&mut self) -> io::Result<(u8, u8)> {
read_magic(&mut self.inner).await?;
read_format_version(&mut self.inner).await
}
/// Reads the raw VCF header.
///
/// The position of the stream is expected to be directly after the file format.
///
/// This returns the raw VCF header as a [`String`]. It can subsequently be parsed as a
/// [`noodles_vcf::Header`].
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bcf as bcf;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bcf").await.map(bcf::AsyncReader::new)?;
/// reader.read_file_format().await?;
///
/// let header = reader.read_header().await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_header(&mut self) -> io::Result<String> {
read_header(&mut self.inner).await
}
/// Reads a single record without decoding (most of) its feilds.
///
/// The stream is expected to be directly after the header or at the start of another record.
///
/// It is more ergonomic to read records using a stream (see [`Self::lazy_records`]), but using
/// this method directly allows the reuse of a single [`lazy::Record`] buffer.
///
/// If successful, the record size is returned. If a record size of 0 is returned, the stream
/// reached EOF.
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bcf as bcf;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bcf").await.map(bcf::AsyncReader::new)?;
/// reader.read_file_format().await?;
/// reader.read_header().await?;
///
/// let mut record = bcf::lazy::Record::default();
/// reader.read_lazy_record(&mut record).await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_lazy_record(&mut self, record: &mut lazy::Record) -> io::Result<usize> {
read_lazy_record(&mut self.inner, &mut self.buf, record).await
}
/// Returns an (async) stream over lazy records starting from the current (input) stream
/// position.
///
/// The (input) stream is expected to be directly after the header or at the start of another
/// record.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use futures::TryStreamExt;
/// use noodles_bcf as bcf;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bcf").await.map(bcf::AsyncReader::new)?;
/// reader.read_file_format().await?;
/// reader.read_header().await?;
///
/// let mut records = reader.lazy_records();
///
/// while let Some(record) = records.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub fn lazy_records(&mut self) -> impl Stream<Item = io::Result<lazy::Record>> + '_ {
Box::pin(stream::try_unfold(
(&mut self.inner, Vec::new(), lazy::Record::default()),
|(mut reader, mut buf, mut record)| async {
read_lazy_record(&mut reader, &mut buf, &mut record)
.await
.map(|n| match n {
0 => None,
_ => Some((record.clone(), (reader, buf, record))),
})
},
))
}
}
impl<R> Reader<bgzf::AsyncReader<R>>
where
R: AsyncRead + Unpin,
{
/// Creates an async BCF reader.
///
/// # Examples
///
/// ```
/// use noodles_bcf as bcf;
/// let data = [];
/// let reader = bcf::AsyncReader::new(&data[..]);
/// ```
pub fn new(inner: R) -> Self {
Self::from(bgzf::AsyncReader::new(inner))
}
/// Returns the current virtual position of the underlying BGZF reader.
///
/// # Examples
///
/// ```
/// use noodles_bcf as bcf;
/// use noodles_bgzf as bgzf;
///
/// let data = Vec::new();
/// let reader = bcf::AsyncReader::new(&data[..]);
/// let virtual_position = reader.virtual_position();
///
/// assert_eq!(reader.virtual_position(), bgzf::VirtualPosition::from(0));
/// ```
pub fn virtual_position(&self) -> bgzf::VirtualPosition {
self.inner.virtual_position()
}
}
impl<R> Reader<bgzf::AsyncReader<R>>
where
R: AsyncRead + AsyncSeek + Unpin,
{
/// Seeks the underlying BGZF reader to the given virtual position.
///
/// Virtual positions typically come from an associated index file.
///
/// # Examples
///
/// ```
/// # use std::io::{self, Cursor};
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bcf as bcf;
/// use noodles_bgzf as bgzf;
///
/// let data = [];
/// let mut reader = bcf::AsyncReader::new(Cursor::new(data));
///
/// let virtual_position = bgzf::VirtualPosition::default();
/// reader.seek(virtual_position).await?;
/// # Ok(())
/// # }
/// ```
pub async fn seek(&mut self, pos: bgzf::VirtualPosition) -> io::Result<bgzf::VirtualPosition> {
self.inner.seek(pos).await
}
/// Returns a stream over records that intersect the given region.
///
/// # Examples
///
/// ```no_run
/// # #[tokio::main]
/// # async fn main() -> Result<(), Box<dyn std::error::Error>> {
/// use futures::TryStreamExt;
/// use noodles_bcf::{self as bcf, header::StringMaps};
/// use noodles_core::Region;
/// use noodles_csi as csi;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bcf").await.map(bcf::AsyncReader::new)?;
/// reader.read_file_format().await?;
///
/// let string_maps: StringMaps = reader.read_header().await?.parse()?;
///
/// let index = csi::r#async::read("sample.bcf.csi").await?;
/// let region = "sq0:8-13".parse()?;
/// let mut query = reader.query(string_maps.contigs(), &index, ®ion)?;
///
/// while let Some(record) = query.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub fn query(
&mut self,
contig_string_map: &ContigStringMap,
index: &csi::Index,
region: &Region,
) -> io::Result<impl Stream<Item = io::Result<lazy::Record>> + '_> {
use crate::reader::resolve_region;
let reference_sequence_id = resolve_region(contig_string_map, region)?;
let chunks = index.query(reference_sequence_id, region.interval())?;
Ok(query(
self,
chunks,
reference_sequence_id,
region.interval(),
))
}
}
impl<R> From<R> for Reader<R> {
fn from(inner: R) -> Self {
Self {
inner,
buf: Vec::new(),
}
}
}
async fn read_magic<R>(reader: &mut R) -> io::Result<()>
where
R: AsyncRead + Unpin,
{
use crate::MAGIC_NUMBER;
let mut magic = [0; 3];
reader.read_exact(&mut magic).await?;
if magic == MAGIC_NUMBER {
Ok(())
} else {
Err(io::Error::new(
io::ErrorKind::InvalidData,
"invalid BCF header",
))
}
}
async fn read_format_version<R>(reader: &mut R) -> io::Result<(u8, u8)>
where
R: AsyncRead + Unpin,
{
let major_version = reader.read_u8().await?;
let minor_version = reader.read_u8().await?;
Ok((major_version, minor_version))
}
async fn read_header<R>(reader: &mut R) -> io::Result<String>
where
R: AsyncRead + Unpin,
{
let l_text = reader.read_u32_le().await.and_then(|len| {
usize::try_from(len).map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
})?;
let mut buf = vec![0; l_text];
reader.read_exact(&mut buf).await?;
c_str_to_string(&buf)
}
fn c_str_to_string(buf: &[u8]) -> io::Result<String> {
use std::ffi::CStr;
CStr::from_bytes_with_nul(buf)
.map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
.and_then(|c_header| {
c_header
.to_str()
.map(|s| s.into())
.map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
})
}
#[cfg(test)]
mod tests {
use super::*;
#[tokio::test]
async fn test_read_magic() {
let data = b"BCF";
let mut reader = &data[..];
assert!(read_magic(&mut reader).await.is_ok());
let data = [];
let mut reader = &data[..];
assert!(matches!(
read_magic(&mut reader).await,
Err(ref e) if e.kind() == io::ErrorKind::UnexpectedEof
));
let data = b"BAM";
let mut reader = &data[..];
assert!(matches!(
read_magic(&mut reader).await,
Err(ref e) if e.kind() == io::ErrorKind::InvalidData
));
}
#[tokio::test]
async fn test_read_format_version() -> io::Result<()> {
let data = [0x02, 0x01];
let mut reader = &data[..];
assert_eq!(read_format_version(&mut reader).await?, (2, 1));
Ok(())
}
#[tokio::test]
async fn test_read_header() -> io::Result<()> {
let data = [
0x08, 0x00, 0x00, 0x00, // l_text = 8
0x6e, 0x6f, 0x6f, 0x64, 0x6c, 0x65, 0x73, 0x00, // text = b"noodles\x00"
];
let mut reader = &data[..];
assert_eq!(read_header(&mut reader).await?, "noodles");
Ok(())
}
}