[](https://circleci.com/gh/onecodex/needletail)
[](https://crates.io/crates/needletail)
# Needletail
Needletail is a MIT-licensed, minimal-copying FASTA/FASTQ parser and _k_-mer processing library for Rust.
The goal is to write a fast *and* well-tested set of functions that more specialized bioinformatics programs can use.
Needletail's goal is to be as fast as the [readfq](https://github.com/lh3/readfq) C library at parsing FASTX files and much (i.e. 25 times) faster than equivalent Python implementations at _k_-mer counting.
# Example
```rust
extern crate needletail;
use needletail::{parse_sequence_path, Sequence};
use std::env;
fn main() {
let filename: String = env::args().nth(1).unwrap();
let mut n_bases = 0;
let mut n_valid_kmers = 0;
parse_sequence_path(
filename,
|_| {},
|seq| {
// seq.id is the name of the record
// seq.seq is the base sequence
// seq.qual is an optional quality score
// keep track of the total number of bases
n_bases += seq.seq.len();
// normalize to make sure all the bases are consistantly capitalized
let norm_seq = seq.normalize(false);
// we make a reverse complemented copy of the sequence first for
// `canonical_kmers` to draw the complemented sequences from.
let rc = norm_seq.reverse_complement();
// now we keep track of the number of AAAAs (or TTTTs via
// canonicalization) in the file; note we also get the postion (i.0;
// in the event there were `N`-containing kmers that were skipped)
// and whether the sequence was complemented (i.2) in addition to
// the canonical kmer (i.1)
for (_, kmer, _) in norm_seq.canonical_kmers(4, &rc) {
if kmer == b"AAAA" {
n_valid_kmers += 1;
}
}
},
)
.expect("parsing failed");
println!("There are {} bases in your file.", n_bases);
println!("There are {} AAAAs in your file.", n_valid_kmers);
}
```
# Installation
Needletail requires `rust` and `cargo` to be installed.
Please use either your local package manager (`homebrew`, `apt-get`, `pacman`, etc) or install these via [rustup](https://www.rustup.rs/).
Once you have Rust set up, you can include needletail in your `Cargo.toml` file like:
```shell
[dependencies]
needletail = "^0.3.1"
```
To install needletail itself for development:
```shell
git clone https://github.com/onecodex/needletail
cargo test # to run tests
```
# Getting Help
Questions are best directed as GitHub issues. We plan to add more documentation soon, but in the meantime "doc" comments are included in the source.
# Contributing
Please do! We're happy to discuss possible additions and/or accept pull requests.