koth-ms

The koth-ms crate (binaries koth_ff and koth_align): fast, memory-efficient peptide feature detection for LC–MS data, written in Rust. Reads mzML (including streamed .mzML.gz), Bruker timsTOF .d with Bruker-calibrated 1/K0, and Thermo .raw through a pure-Rust reader (no .NET), all in the default build.

cargo install --locked koth-ms
koth_ff sample.mzML --output results/

Installs both koth_ff and koth_align. Feature finding writes chromatographic traces, isotope features, a summary, and the resolved configuration. The alignment tool produces multi-run intensity matrices; its q-values are exploratory confidence measures, not calibrated peptide-identification FDR.

Documentation · Benchmarks · Source and issues · Citation

Licensed under MIT.