kfb2zarr

Convert KFBio whole slide images (.kfb, .kfbf) to OME-Zarr.

Installation

Requires Rust.

cargo install kfb2zarr

Usage

kfb2zarr slide.kfb slide.ome.zarr

To replace an existing output store:

kfb2zarr slide.kfb slide.ome.zarr --overwrite

Viewing the output

The output .ome.zarr can be viewed locally with:

• QuPath ≥ 0.7.0 via drag & drop
• napari via the napari-ome-zarr plugin

Output has been spot-checked in QuPath and vizarr against the same files opened in KFBio's KFSlideOS viewer.

Acknowledgements

Sample data used during development was kindly provided by KFBio. kfb2zarr is not an official product of KFBio.

kfb2zarr writes Zarr stores with:

Deakin, L. (2025). zarrs: A High-Performance Rust Library for the Zarr Array Storage Format (Version 0.22.10) [Computer software]. https://github.com/zarrs/zarrs