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//! PerkinElmer/Evotec FLEX HCS format reader.
//!
//! FLEX is a TIFF-based format used for high-content screening (HCS) by
//! PerkinElmer/Evotec. Each FLEX image plane stores an XML metadata block in a
//! custom TIFF tag (65200). That XML contains one `<Array>` element per image
//! plane carrying `Name` and `Factor` attributes. `Factor` is a per-plane
//! scaling multiplier that must be applied to pixel values on read; when any
//! factor is greater than 1 the effective pixel type widens (UINT16, or UINT32
//! when the largest factor exceeds 256). This mirrors `FlexReader.java`.
//!
//! A FLEX dataset is normally a *directory* of `.flex` files, one per
//! (well, field), optionally accompanied by `.mea`/`.res` measurement
//! companions that enumerate the wells/fields. Following `FlexReader.java`:
//! - `.flex` filenames of the form `nnnnnnnnn.flex` (14 chars) encode the
//! well row (chars 0..3), well column (chars 3..6) and field (chars 6..9),
//! all 1-based.
//! - Files are grouped by well; each file within a well is a field.
//! - One OME series is produced per (plate, well, field).
//! - The `.mea` file lists `<Picture path=...>` entries pointing at the
//! `.flex` files; the `.res` file carries the plate acquisition date.
//!
//! When no companion files are present and the single `.flex` cannot be grouped
//! (filename is not the 14-char well pattern), the reader falls back to the
//! original single-file behavior: the TIFF IFDs map directly to planes of a
//! single series.
//!
//! This reader wraps `TiffReader` for the raw TIFF pixel I/O and layers the
//! Flex XML factor parsing + pixel scaling + well/field series assembly on top.
use crate::common::error::{BioFormatsError, Result};
use crate::common::metadata::ImageMetadata;
use crate::common::ome_metadata::{create_lsid, OmeMetadata, OmePlate, OmeWell, OmeWellSample};
use crate::common::pixel_type::PixelType;
use crate::common::reader::FormatReader;
use crate::tiff::ifd::IfdValue;
use std::path::{Path, PathBuf};
/// Custom Flex IFD entry holding the per-image XML (FlexReader.FLEX = 65200).
const FLEX_TAG: u16 = 65200;
/// One `.flex` file in a grouped dataset: a (well row, well column, field).
struct FlexFile {
row: u32,
column: u32,
field: u32,
path: PathBuf,
/// Per-plane scaling factors for this file (`None` when every factor is 1).
factors: Option<Vec<f64>>,
}
pub struct FlexReader {
/// TiffReader bound to the *currently selected* series' `.flex` file.
inner: crate::tiff::TiffReader,
/// Path currently loaded into `inner` (so we avoid re-opening on set_series).
inner_path: Option<PathBuf>,
/// Grouped files (multi-file HCS mode). Empty in single-file fallback.
flex_files: Vec<FlexFile>,
/// Current series index.
series: usize,
/// Effective pixel type after factor widening (applies to series 0 like Java).
scaled_pixel_type: Option<PixelType>,
/// Per-series metadata (cloned from the representative file's TIFF series 0).
series_meta: Vec<ImageMetadata>,
/// planes-per-image-count for the dataset (Z*C*T of series 0).
image_count: u32,
/// HCS layout.
plate_count: u32,
well_count: u32,
field_count: u32,
well_rows: u32,
well_columns: u32,
/// (row, col) for each well index (parallel to the column-major well list).
well_number: Vec<(u32, u32)>,
/// Companion measurement files found (.mea/.res), absolute paths.
measurement_files: Vec<PathBuf>,
/// Plate acquisition start time from the .res file.
plate_acq_start_time: Option<String>,
/// Plate name / barcode parsed from XML.
plate_name: Option<String>,
plate_barcode: Option<String>,
/// True when running in single-file fallback mode.
single_file: bool,
}
impl FlexReader {
pub fn new() -> Self {
FlexReader {
inner: crate::tiff::TiffReader::new(),
inner_path: None,
flex_files: Vec::new(),
series: 0,
scaled_pixel_type: None,
series_meta: Vec::new(),
image_count: 0,
plate_count: 0,
well_count: 0,
field_count: 0,
well_rows: 0,
well_columns: 0,
well_number: Vec::new(),
measurement_files: Vec::new(),
plate_acq_start_time: None,
plate_name: None,
plate_barcode: None,
single_file: true,
}
}
/// Extract the Flex XML block (tag 65200) from the first IFD as a string.
fn flex_xml(&self) -> Option<String> {
let ifd = self.inner.ifd(0)?;
match ifd.get(FLEX_TAG) {
Some(IfdValue::Ascii(s)) => Some(s.clone()),
Some(IfdValue::Byte(b)) | Some(IfdValue::Undefined(b)) => {
Some(String::from_utf8_lossy(b).into_owned())
}
_ => None,
}
}
/// Ensure `inner` is bound to the `.flex` file for series `s`.
fn bind_series(&mut self, s: usize) -> Result<()> {
if self.single_file {
self.series = s;
return Ok(());
}
let file = self
.flex_files
.get(self.file_index_for_series(s))
.ok_or(BioFormatsError::SeriesOutOfRange(s))?;
let path = file.path.clone();
if self.inner_path.as_deref() != Some(path.as_path()) {
self.inner.set_id(&path)?;
self.inner_path = Some(path);
}
self.series = s;
Ok(())
}
/// Map an OME series index to an index into `flex_files`.
///
/// Mirrors Java `lookupFile(int fileSeries)`: lengths =
/// {fieldCount, wellCount, plateCount}, raster-to-position, then look up by
/// (row, col, field).
fn file_index_for_series(&self, series: usize) -> usize {
if self.flex_files.len() == 1 {
return 0;
}
let field_count = self.field_count.max(1);
let well_count = self.well_count.max(1);
let plate_count = self.plate_count.max(1);
// effectiveFieldCount: 1 when wellCount*plateCount == files.
let effective_field_count = if (well_count * plate_count) as usize == self.flex_files.len()
{
1
} else {
field_count
};
let lengths = [
field_count as usize,
well_count as usize,
plate_count as usize,
];
let pos = raster_to_position(&lengths, series);
let zero_well = well_count == 1 && effective_field_count == 1;
let (row, col) = if zero_well {
(0, 0)
} else {
self.well_number.get(pos[1]).copied().unwrap_or((0, 0))
};
let field = if effective_field_count == 1 {
0
} else {
pos[0] as u32
};
self.flex_files
.iter()
.position(|f| f.row == row && f.column == col && f.field == field)
.unwrap_or(0)
}
/// Parse `<Array Name=.. Factor=..>` arrays and derive factors / widening.
/// Returns the factor vector (`None` when all factors are 1). The scaled
/// pixel type is only widened from the first file's factors: Java derives
/// `core.get(0).pixelType` from file-0's max factor (FlexReader.java:909),
/// so a non-first file must not widen it. Pass `update_pixel_type = false`
/// for files other than the first.
fn derive_factors(
&mut self,
total_planes: usize,
update_pixel_type: bool,
) -> Result<Option<Vec<f64>>> {
if total_planes == 0 {
return Err(BioFormatsError::Format(
"Flex: TIFF file has no image planes".into(),
));
}
let Some(mut xml) = self.flex_xml() else {
return Ok(None);
};
let trimmed = xml.trim();
if trimmed.ends_with(">>") || trimmed.ends_with('%') {
xml = trimmed[..trimmed.len() - 1].to_string();
} else {
xml = trimmed.to_string();
}
let (_names, factors) = parse_flex_arrays(&xml);
if !factors.is_empty() && factors.len() != total_planes {
return Err(BioFormatsError::Format(format!(
"Flex: XML Array count {} does not match TIFF plane count {}",
factors.len(),
total_planes
)));
}
let mut factor_values = vec![1.0f64; total_planes];
let mut max_idx = 0usize;
let mut one_factors = true;
for (i, f) in factors.iter().enumerate() {
let q = f.parse::<f64>().map_err(|_| {
BioFormatsError::Format(format!("Flex: invalid Array Factor {f:?}"))
})?;
if !q.is_finite() || q <= 0.0 {
return Err(BioFormatsError::Format(format!(
"Flex: invalid Array Factor {f:?}"
)));
}
if i < factor_values.len() {
factor_values[i] = q;
if q > factor_values[max_idx] {
max_idx = i;
}
if q != 1.0 {
one_factors = false;
}
}
}
if update_pixel_type {
let max_factor = factor_values.get(max_idx).copied().unwrap_or(1.0);
if max_factor > 256.0 {
self.scaled_pixel_type = Some(PixelType::Uint32);
} else if max_factor > 1.0 {
self.scaled_pixel_type = Some(PixelType::Uint16);
}
}
if one_factors {
Ok(None)
} else {
Ok(Some(factor_values))
}
}
/// Apply the Flex pixel scaling factor to a freshly read plane, widening to
/// `scaled_pixel_type` if needed. Mirrors `FlexReader.openBytes` scaling.
fn apply_factor(&self, raw: Vec<u8>, plane: u32, little_endian: bool) -> Vec<u8> {
let src_pt = self
.inner
.series_list()
.get(self.inner.series())
.map(|s| s.metadata.pixel_type)
.unwrap_or(PixelType::Uint8);
let n_bytes = src_pt.bytes_per_sample();
let dst_pt = self.scaled_pixel_type.unwrap_or(src_pt);
let bpp = dst_pt.bytes_per_sample();
let factor = if self.single_file {
// factors stored on inner-file order via derive on series 0.
self.series_factor(0, plane)
} else {
let fi = self.file_index_for_series(self.series);
self.series_factor(fi, plane)
};
if factor == 1.0 && n_bytes == bpp {
return raw;
}
if n_bytes == 0 || bpp == 0 {
return raw;
}
let num = raw.len() / n_bytes;
let mut out = vec![0u8; num * bpp];
for i in 0..num {
let q = read_uint(&raw, i * n_bytes, n_bytes, little_endian);
let scaled = (q as f64 * factor) as u64;
write_uint(&mut out, i * bpp, bpp, scaled, little_endian);
}
out
}
fn series_factor(&self, file_index: usize, plane: u32) -> f64 {
self.flex_files
.get(file_index)
.and_then(|f| f.factors.as_ref())
.and_then(|v| v.get(plane as usize).copied())
.unwrap_or(1.0)
}
}
impl Default for FlexReader {
fn default() -> Self {
Self::new()
}
}
/// FormatTools.rasterToPosition: convert a raster index to per-axis positions
/// (axis 0 fastest-varying), mirroring the Java helper.
fn raster_to_position(lengths: &[usize], mut raster: usize) -> Vec<usize> {
let mut pos = vec![0usize; lengths.len()];
for (i, &len) in lengths.iter().enumerate() {
let len = len.max(1);
pos[i] = raster % len;
raster /= len;
}
pos
}
/// Read an unsigned integer of `n` bytes from `buf` at `off`.
fn read_uint(buf: &[u8], off: usize, n: usize, little_endian: bool) -> u64 {
let mut v = 0u64;
for i in 0..n {
let byte = buf.get(off + i).copied().unwrap_or(0) as u64;
if little_endian {
v |= byte << (8 * i);
} else {
v = (v << 8) | byte;
}
}
v
}
/// Write an unsigned integer of `n` bytes into `buf` at `off`.
fn write_uint(buf: &mut [u8], off: usize, n: usize, value: u64, little_endian: bool) {
for i in 0..n {
let shift = if little_endian {
8 * i
} else {
8 * (n - 1 - i)
};
if let Some(slot) = buf.get_mut(off + i) {
*slot = ((value >> shift) & 0xff) as u8;
}
}
}
/// Parse all `<Array ... Name=... Factor=...>` elements, returning the lists of
/// names and factor strings in document order (mirrors FlexHandler).
fn parse_flex_arrays(xml: &str) -> (Vec<String>, Vec<String>) {
let mut names = Vec::new();
let mut factors = Vec::new();
let bytes = xml.as_bytes();
let mut i = 0;
while let Some(rel) = xml[i..].find("<Array") {
let start = i + rel;
let end = match xml[start..].find('>') {
Some(e) => start + e,
None => break,
};
let tag = &xml[start..end];
if let Some(name) = xml_attr(tag, "Name") {
names.push(name);
}
if let Some(factor) = xml_attr(tag, "Factor") {
factors.push(factor);
}
i = end + 1;
if i >= bytes.len() {
break;
}
}
(names, factors)
}
/// Extract an XML attribute value (`attr="value"`) from a single start tag.
fn xml_attr(tag: &str, attr: &str) -> Option<String> {
let mut search_from = 0;
while let Some(rel) = tag[search_from..].find(attr) {
let pos = search_from + rel;
let prev_ok = pos == 0 || tag.as_bytes()[pos - 1].is_ascii_whitespace();
let after = pos + attr.len();
let rest = tag[after..].trim_start();
if prev_ok && rest.starts_with('=') {
let rest = rest[1..].trim_start();
let quote = rest.chars().next()?;
if quote == '"' || quote == '\'' {
let val_start = 1;
if let Some(end) = rest[val_start..].find(quote) {
return Some(rest[val_start..val_start + end].to_string());
}
}
}
search_from = after;
}
None
}
/// Extract the text content of the first `<tag>..</tag>` (or `Barcode` style)
/// occurrence. Used for plate Barcode/PlateName from the Flex XML.
fn xml_element_text(xml: &str, name: &str) -> Option<String> {
let needle = name;
let idx = xml.find(needle)?;
let start = xml[idx..].find('>').map(|e| idx + e + 1)?;
let end = xml[idx..].find('<').map(|e| idx + e)?;
if end > start {
Some(xml[start..end].to_string())
} else {
None
}
}
/// Parse the well row/column (0-based) from a 14-char `nnnnnnnnn.flex` name.
/// Returns None if the name does not match the pattern.
fn parse_well(name: &str) -> Option<(u32, u32)> {
if name.len() == 14 && name.to_ascii_lowercase().ends_with(".flex") {
let row = name.get(0..3)?.parse::<u32>().ok()?;
let col = name.get(3..6)?.parse::<u32>().ok()?;
return Some((row.saturating_sub(1), col.saturating_sub(1)));
}
None
}
/// Parse the field index (0-based) from a 14-char `nnnnnnnnn.flex` name.
fn parse_field(name: &str) -> u32 {
if name.len() == 14 && name.to_ascii_lowercase().ends_with(".flex") {
if let Some(s) = name.get(6..9) {
if let Ok(v) = s.parse::<u32>() {
return v.saturating_sub(1);
}
}
}
0
}
/// Parse a `.mea` file's `<Picture path=...>` entries into a list of `.flex`
/// file names (relative). Mirrors MeaHandler (minus server-name remapping,
/// which is not applicable without a configured server map).
fn parse_mea_flex_names(text: &str) -> Vec<String> {
let mut out = Vec::new();
let mut i = 0;
while let Some(rel) = text[i..].find("<Picture") {
let start = i + rel;
let end = match text[start..].find('>') {
Some(e) => start + e,
None => break,
};
let tag = &text[start..end];
if let Some(mut path) = xml_attr(tag, "path") {
if !path.to_ascii_lowercase().ends_with(".flex") {
path.push_str(".flex");
}
// Normalise separators to native; we only use the file name below.
let path = path.replace('\\', "/");
out.push(path);
}
i = end + 1;
}
out
}
/// Parse the plate acquisition date attribute from a `.res` file
/// (`<AnalysisResults date="...">`). Mirrors ResHandler.
fn parse_res_date(text: &str) -> Option<String> {
let idx = text.find("<AnalysisResults")?;
let end = text[idx..].find('>').map(|e| idx + e)?;
let tag = &text[idx..end];
xml_attr(tag, "date")
}
/// Find companion `.mea`/`.res` files in the same directory as the `.flex`.
fn find_measurement_files(flex_path: &Path) -> Vec<PathBuf> {
let mut out = Vec::new();
let Some(dir) = flex_path.parent() else {
return out;
};
if let Ok(rd) = std::fs::read_dir(dir) {
for entry in rd.flatten() {
let p = entry.path();
let ext = p
.extension()
.and_then(|e| e.to_str())
.map(|e| e.to_ascii_lowercase());
if matches!(ext.as_deref(), Some("mea") | Some("res")) {
out.push(p);
}
}
}
out.sort();
out
}
/// Collect grouped `.flex` files for a dataset. Returns the sorted list of
/// 14-char-pattern `.flex` files in the same directory, or just the input file
/// when no grouping is possible.
fn collect_flex_files(flex_path: &Path) -> Vec<PathBuf> {
let name = flex_path
.file_name()
.and_then(|n| n.to_str())
.unwrap_or_default();
// Only group when the file follows the nnnnnnnnn.flex naming convention.
if parse_well(name).is_none() {
return vec![flex_path.to_path_buf()];
}
let Some(dir) = flex_path.parent() else {
return vec![flex_path.to_path_buf()];
};
let mut files: Vec<PathBuf> = Vec::new();
if let Ok(rd) = std::fs::read_dir(dir) {
for entry in rd.flatten() {
let p = entry.path();
let n = p.file_name().and_then(|x| x.to_str()).unwrap_or_default();
if n.len() == 14 && n.to_ascii_lowercase().ends_with(".flex") {
files.push(p);
}
}
}
if files.is_empty() {
files.push(flex_path.to_path_buf());
}
files.sort();
files
}
impl FormatReader for FlexReader {
fn is_this_type_by_name(&self, path: &Path) -> bool {
path.extension()
.and_then(|e| e.to_str())
.map(|e| {
e.eq_ignore_ascii_case("flex")
|| e.eq_ignore_ascii_case("mea")
|| e.eq_ignore_ascii_case("res")
})
.unwrap_or(false)
}
fn is_this_type_by_bytes(&self, header: &[u8]) -> bool {
if header.len() < 4 {
return false;
}
(header[0] == 0x49 && header[1] == 0x49 && header[2] == 0x2A && header[3] == 0x00)
|| (header[0] == 0x4D && header[1] == 0x4D && header[2] == 0x00 && header[3] == 0x2A)
|| (header[0] == 0x49 && header[1] == 0x49 && header[2] == 0x2B && header[3] == 0x00)
}
fn set_id(&mut self, path: &Path) -> Result<()> {
let _ = self.close();
let result: Result<()> = (|| {
// Resolve the .flex entry point. If handed a .mea/.res, find a .flex in
// the same directory (Java initMeaFile/initResFile fall back to this).
let ext = path
.extension()
.and_then(|e| e.to_str())
.map(|e| e.to_ascii_lowercase());
let flex_entry: PathBuf = if matches!(ext.as_deref(), Some("flex")) {
path.to_path_buf()
} else {
// .mea / .res: locate a .flex in the same directory.
let dir = path.parent().unwrap_or_else(|| Path::new("."));
let mut found = None;
if let Ok(rd) = std::fs::read_dir(dir) {
let mut candidates: Vec<PathBuf> = rd
.flatten()
.map(|e| e.path())
.filter(|p| {
p.extension()
.and_then(|e| e.to_str())
.map(|e| e.eq_ignore_ascii_case("flex"))
.unwrap_or(false)
})
.collect();
candidates.sort();
found = candidates.into_iter().next();
}
found.ok_or_else(|| {
BioFormatsError::UnsupportedFormat(
"Flex .mea/.res companion has no .flex files in its directory".into(),
)
})?
};
let measurement_files = find_measurement_files(&flex_entry);
// Parse .res for the plate acquisition start time.
for m in &measurement_files {
if m.extension()
.and_then(|e| e.to_str())
.map(|e| e.eq_ignore_ascii_case("res"))
.unwrap_or(false)
{
if let Ok(text) = std::fs::read_to_string(m) {
if let Some(d) = parse_res_date(&text) {
self.plate_acq_start_time = Some(d);
}
}
}
}
// Determine the grouped file list. Prefer the .mea list when present.
let mut grouped: Vec<PathBuf> = Vec::new();
for m in &measurement_files {
if m.extension()
.and_then(|e| e.to_str())
.map(|e| e.eq_ignore_ascii_case("mea"))
.unwrap_or(false)
{
if let Ok(text) = std::fs::read_to_string(m) {
let dir = flex_entry.parent().unwrap_or_else(|| Path::new("."));
for rel in parse_mea_flex_names(&text) {
// Match by file name within the .flex directory.
let fname = rel.rsplit('/').next().unwrap_or(&rel);
let candidate = dir.join(fname);
if candidate.exists() {
grouped.push(candidate);
}
}
}
}
}
if grouped.is_empty() {
grouped = collect_flex_files(&flex_entry);
} else {
grouped.sort();
grouped.dedup();
}
self.measurement_files = measurement_files;
// Single-file fallback: cannot group by well pattern.
let entry_name = flex_entry
.file_name()
.and_then(|n| n.to_str())
.unwrap_or_default();
let groupable = grouped.len() > 1 || parse_well(entry_name).is_some();
if !groupable {
// ---- single-file mode (original behavior) ----
self.single_file = true;
self.inner.set_id(&flex_entry)?;
self.inner_path = Some(flex_entry.clone());
let total_planes: usize = (0..self.inner.series_count())
.map(|s| self.inner.series_list()[s].metadata.image_count as usize)
.sum();
let factors = self.derive_factors(total_planes, true)?;
if let Some(pt) = self.scaled_pixel_type {
let series = self.inner.series_list_mut();
if let Some(s0) = series.first_mut() {
s0.metadata.pixel_type = pt;
s0.metadata.bits_per_pixel = (pt.bytes_per_sample() * 8) as u8;
}
}
// store the single file's factors as flex_files[0] for apply_factor.
self.flex_files = vec![FlexFile {
row: 0,
column: 0,
field: 0,
path: flex_entry,
factors,
}];
self.series = 0;
self.image_count = self
.inner
.series_list()
.first()
.map(|s| s.metadata.image_count)
.unwrap_or(0);
return Ok(());
}
// ---- multi-file HCS mode ----
self.single_file = false;
// Group files by well (row, col), each file within a well is a field.
// Build well list in (row, col) order; record well_number layout.
use std::collections::BTreeMap;
let mut wells: BTreeMap<(u32, u32), Vec<PathBuf>> = BTreeMap::new();
let mut max_row = 0u32;
let mut max_col = 0u32;
for f in &grouped {
let n = f.file_name().and_then(|x| x.to_str()).unwrap_or_default();
let (row, col) = if grouped.len() == 1 {
(0, 0)
} else {
parse_well(n).unwrap_or((0, 0))
};
max_row = max_row.max(row);
max_col = max_col.max(col);
wells.entry((row, col)).or_default().push(f.clone());
}
self.well_rows = max_row + 1;
self.well_columns = max_col + 1;
if grouped.len() == 1 {
self.well_rows = 1;
self.well_columns = 1;
}
self.well_count = wells.len() as u32;
// Build the flex_files list in well order, fields sorted within a well.
let mut flex_files: Vec<FlexFile> = Vec::new();
let mut well_number: Vec<(u32, u32)> = Vec::new();
let mut n_files_per_well = 1usize;
let mut expected_files_per_well: Option<usize> = None;
for (&(row, col), files) in &wells {
well_number.push((row, col));
let mut sorted = files.clone();
sorted.sort();
if let Some(expected) = expected_files_per_well {
if sorted.len() != expected {
return Err(BioFormatsError::Format(format!(
"Flex: inconsistent field count for well ({row},{col}): got {}, expected {expected}",
sorted.len()
)));
}
} else {
expected_files_per_well = Some(sorted.len());
}
n_files_per_well = sorted.len();
// Java assigns the field index by sorted position within the well
// (FlexFile.field = field loop variable), but the filename's field
// digits (chars 6..9) are the authoritative field number. Use the
// filename field when the 14-char pattern is present, falling back
// to sorted position otherwise.
for (pos, p) in sorted.into_iter().enumerate() {
let n = p.file_name().and_then(|x| x.to_str()).unwrap_or_default();
let field = if n.len() == 14 {
parse_field(n)
} else {
pos as u32
};
flex_files.push(FlexFile {
row,
column: col,
field,
path: p,
factors: None,
});
}
}
self.well_number = well_number;
// Parse the first file to obtain core dimensions + factors.
let first_path = flex_files[0].path.clone();
self.inner.set_id(&first_path)?;
self.inner_path = Some(first_path);
let n_planes = self
.inner
.series_list()
.first()
.map(|s| s.metadata.image_count)
.unwrap_or(0);
if n_planes == 0 {
return Err(BioFormatsError::Format(
"Flex: first grouped file has no image planes".into(),
));
}
// Derive factors & widening from series-0 XML. Only the first file
// sets the scaled pixel type (Java FlexReader.java:909).
let factors = self.derive_factors(n_planes as usize, true)?;
flex_files[0].factors = factors;
// populateCoreMetadata field logic (faithful to the common case):
// each file holds exactly imageCount planes => one field per file, so
// fieldCount = number of files per well (Java: `fieldCount *= nFiles`
// when fieldCount == 1). Fields-stored-within-a-file is rare and not
// reconstructed here.
let field_count = (n_files_per_well as u32).max(1);
self.field_count = field_count;
self.image_count = n_planes;
self.plate_count = 1;
// seriesCount = plateCount * wellCount * fieldCount.
let series_count =
(self.plate_count * self.well_count * self.field_count).max(1) as usize;
// Apply factor widening to the (single) base metadata.
let mut base_meta = self
.inner
.series_list()
.first()
.map(|s| s.metadata.clone())
.ok_or_else(|| BioFormatsError::Format("Flex: no IFDs in first file".into()))?;
if let Some(pt) = self.scaled_pixel_type {
base_meta.pixel_type = pt;
base_meta.bits_per_pixel = (pt.bytes_per_sample() * 8) as u8;
}
// Parse factors for the remaining files (each file may have its own XML).
for i in 1..flex_files.len() {
let p = flex_files[i].path.clone();
self.inner.set_id(&p)?;
self.inner_path = Some(p.clone());
let np = self
.inner
.series_list()
.first()
.map(|s| s.metadata.image_count)
.unwrap_or(n_planes);
if np != n_planes {
return Err(BioFormatsError::Format(format!(
"Flex: grouped file {} has {} planes, expected {}",
p.display(),
np,
n_planes
)));
}
// Non-first files contribute their own per-plane factors but must
// not widen the scaled pixel type (derived from file 0 only).
let f = self.derive_factors(np as usize, false)?;
flex_files[i].factors = f;
}
// Plate name/barcode from the first file's XML.
if let Some(xml) = {
// rebind to first file to read its XML
let p = flex_files[0].path.clone();
if self.inner_path.as_deref() != Some(p.as_path()) {
self.inner.set_id(&p)?;
self.inner_path = Some(p);
}
self.flex_xml()
} {
self.plate_barcode = xml_element_text(&xml, "Barcode");
self.plate_name = xml_element_text(&xml, "PlateName");
}
self.flex_files = flex_files;
self.series_meta = vec![base_meta; series_count];
self.series = 0;
// Bind inner to series 0's file.
self.bind_series(0)?;
Ok(())
})();
if result.is_err() {
let _ = self.close();
}
result
}
fn close(&mut self) -> Result<()> {
self.flex_files.clear();
self.inner_path = None;
self.scaled_pixel_type = None;
self.series_meta.clear();
self.well_number.clear();
self.measurement_files.clear();
self.plate_acq_start_time = None;
self.plate_name = None;
self.plate_barcode = None;
self.series = 0;
self.single_file = true;
self.inner.close()
}
fn series_count(&self) -> usize {
if self.single_file {
self.inner.series_count()
} else {
self.series_meta.len()
}
}
fn set_series(&mut self, s: usize) -> Result<()> {
if self.single_file {
if self.inner.series_count() == 0 {
return Err(BioFormatsError::NotInitialized);
}
self.inner.set_series(s)?;
self.series = s;
Ok(())
} else {
if s >= self.series_meta.len() {
return Err(BioFormatsError::SeriesOutOfRange(s));
}
self.bind_series(s)
}
}
fn series(&self) -> usize {
self.series
}
fn metadata(&self) -> &ImageMetadata {
if self.single_file {
self.inner.metadata()
} else {
self.series_meta
.get(self.series)
.unwrap_or(crate::common::reader::uninitialized_metadata())
}
}
fn open_bytes(&mut self, p: u32) -> Result<Vec<u8>> {
if !self.single_file {
self.bind_series(self.series)?;
}
let le = self.inner.is_little_endian();
let raw = self.inner.open_bytes(p)?;
Ok(self.apply_factor(raw, p, le))
}
fn open_bytes_region(&mut self, p: u32, x: u32, y: u32, w: u32, h: u32) -> Result<Vec<u8>> {
if !self.single_file {
self.bind_series(self.series)?;
}
let le = self.inner.is_little_endian();
let raw = self.inner.open_bytes_region(p, x, y, w, h)?;
Ok(self.apply_factor(raw, p, le))
}
fn open_thumb_bytes(&mut self, p: u32) -> Result<Vec<u8>> {
if !self.single_file {
self.bind_series(self.series)?;
}
self.inner.open_thumb_bytes(p)
}
fn resolution_count(&self) -> usize {
self.inner.resolution_count()
}
fn set_resolution(&mut self, l: usize) -> Result<()> {
self.inner.set_resolution(l)
}
fn resolution(&self) -> usize {
self.inner.resolution()
}
fn ome_metadata(&self) -> Option<OmeMetadata> {
if std::ptr::eq(
self.metadata(),
crate::common::reader::uninitialized_metadata(),
) {
return None;
}
let mut ome = OmeMetadata::from_image_metadata(self.metadata());
if self.single_file {
return Some(ome);
}
// Build one Image per series; one Plate with Wells/WellSamples.
let series_count = self.series_meta.len();
ome.images = (0..series_count)
.map(|_| crate::common::ome_metadata::OmeImage::default())
.collect();
let field_count = self.field_count.max(1) as usize;
let well_count = self.well_count.max(1) as usize;
let plate_count = self.plate_count.max(1) as usize;
let lengths = [field_count, well_count, plate_count];
let mut plate = OmePlate {
id: Some(create_lsid("Plate", &[0])),
name: self
.plate_name
.clone()
.or_else(|| Some("Plate".to_string())),
rows: self.well_rows,
columns: self.well_columns,
wells: Vec::new(),
};
if let Some(barcode) = &self.plate_barcode {
plate.name = Some(match &plate.name {
Some(n) => format!("{barcode} {n}"),
None => barcode.clone(),
});
}
// Index wells by (row,col) -> Vec<WellSample>.
use std::collections::BTreeMap;
let mut well_map: BTreeMap<(u32, u32), Vec<OmeWellSample>> = BTreeMap::new();
for i in 0..series_count {
let pos = raster_to_position(&lengths, i);
let (row, col) = self.well_number.get(pos[1]).copied().unwrap_or((0, 0));
let field = pos[0] as u32;
well_map.entry((row, col)).or_default().push(OmeWellSample {
id: Some(create_lsid(
"WellSample",
&[
pos[2],
(row * self.well_columns + col) as usize,
field as usize,
],
)),
index: i as u32,
image_ref: Some(i),
position_x: None,
position_y: None,
});
}
for ((row, col), samples) in well_map {
plate.wells.push(OmeWell {
id: Some(create_lsid(
"Well",
&[0, (row * self.well_columns + col) as usize],
)),
row,
column: col,
well_samples: samples,
});
}
ome.plates = vec![plate];
Some(ome)
}
}
#[cfg(test)]
mod tests {
use super::*;
#[test]
fn parses_well_and_field_from_14char_name() {
// 002003001.flex -> row 1, col 2, field 0 (all 1-based in file)
assert_eq!(parse_well("002003001.flex"), Some((1, 2)));
assert_eq!(parse_field("002003001.flex"), 0);
assert_eq!(parse_field("002003004.flex"), 3);
}
#[test]
fn non_pattern_name_is_not_groupable() {
assert_eq!(parse_well("test.flex"), None);
assert_eq!(parse_well("image001.flex"), None);
}
#[test]
fn mea_picture_paths_get_flex_extension() {
let mea =
r#"<root><Picture path="dir/002003001"/><Picture path="dir\002003002.flex"/></root>"#;
let names = parse_mea_flex_names(mea);
assert_eq!(names, vec!["dir/002003001.flex", "dir/002003002.flex"]);
}
#[test]
fn res_date_parsed() {
let res = r#"<AnalysisResults date="01.02.2010 10:20:30" foo="bar">"#;
assert_eq!(
parse_res_date(res),
Some("01.02.2010 10:20:30".to_string())
);
}
#[test]
fn raster_to_position_axis0_fastest() {
// lengths {field=2, well=3, plate=1}; series 4 -> field 0, well 2.
let p = raster_to_position(&[2, 3, 1], 4);
assert_eq!(p, vec![0, 2, 0]);
}
#[test]
fn flex_arrays_parse_name_and_factor() {
let xml = r#"<Arrays><Array Name="1_ch1" Factor="2.0"/><Array Name="1_ch2" Factor="1"/></Arrays>"#;
let (names, factors) = parse_flex_arrays(xml);
assert_eq!(names, vec!["1_ch1", "1_ch2"]);
assert_eq!(factors, vec!["2.0", "1"]);
}
}