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//! 4-bit IUPAC nucleotide ambiguity codes
//!
//! IUPAC nucleotide ambiguity codes are represented with 4 bits
//!
//! | | A | C | G | T |
//! | - | - | - | - | - |
//! | A | 1 | 0 | 0 | 0 |
//! | C | 0 | 1 | 0 | 0 |
//! | G | 0 | 0 | 1 | 0 |
//! | T | 0 | 0 | 0 | 1 |
//! | Y | 0 | 1 | 0 | 1 |
//! | R | 1 | 0 | 1 | 0 |
//! | W | 1 | 0 | 0 | 1 |
//! | S | 0 | 1 | 1 | 0 |
//! | K | 0 | 0 | 1 | 1 |
//! | M | 1 | 1 | 0 | 0 |
//! | D | 1 | 0 | 1 | 1 |
//! | V | 1 | 1 | 1 | 0 |
//! | H | 1 | 1 | 0 | 1 |
//! | B | 0 | 1 | 1 | 1 |
//! | N | 1 | 1 | 1 | 1 |
//! | X/- | 0 | 0 | 0 | 0 |
//!
//! This naturally supports set membership operations:
//!
//! ```rust
//! use bio_seq::prelude::*;
//!
//! // Set union:
//! assert_eq!(iupac!("AS-GYTNA") | iupac!("ANTGCAT-"), iupac!("ANTGYWNA"));
//!
//! // Set intersection:
//! assert_eq!(iupac!("ACGTSWKM") & iupac!("WKMSTNNA"), iupac!("A----WKA"));
//! ```
//!
//! Which can be used to implement pattern matching:
//!
//! ```rust
//! use bio_seq::prelude::*;
//!
//! let seq = iupac!("AGCTNNCAGTCGACGTATGTA");
//! let pattern = iupac!("AYG");
//!
//! for slice in seq.windows(pattern.len()) {
//! if pattern.contains(slice) {
//! println!("{slice} matches pattern");
//! }
//! }
//!
//! // ACG matches pattern
//! // ATG matches pattern
//! ```
use crate::codec::{dna::Dna, Codec};
use crate::seq::{Seq, SeqSlice};
use core::ops::{BitAnd, BitOr};
#[derive(Clone, Copy, Debug, PartialEq, Eq, Hash, Codec)]
#[bits(4)]
#[repr(u8)]
pub enum Iupac {
A = 0b1000,
C = 0b0100,
G = 0b0010,
T = 0b0001,
R = 0b1010,
Y = 0b0101,
S = 0b0110,
W = 0b1001,
K = 0b0011,
M = 0b1100,
B = 0b0111,
D = 0b1011,
H = 0b1101,
V = 0b1110,
N = 0b1111,
#[display('-')]
X = 0b0000,
}
impl From<Dna> for Iupac {
fn from(dna: Dna) -> Self {
match dna {
Dna::A => Iupac::A,
Dna::C => Iupac::C,
Dna::G => Iupac::G,
Dna::T => Iupac::T,
}
}
}
impl BitAnd for Seq<Iupac> {
type Output = Self;
fn bitand(self, rhs: Self) -> Self::Output {
self.bit_and(rhs)
}
}
impl BitOr for Seq<Iupac> {
type Output = Self;
fn bitor(self, rhs: Self) -> Self::Output {
self.bit_or(rhs)
}
}
impl BitAnd for &SeqSlice<Iupac> {
type Output = Seq<Iupac>;
fn bitand(self, rhs: Self) -> Self::Output {
self.bit_and(rhs)
}
}
impl BitOr for &SeqSlice<Iupac> {
type Output = Seq<Iupac>;
fn bitor(self, rhs: Self) -> Self::Output {
self.bit_or(rhs)
}
}
impl Seq<Iupac> {
pub fn contains(&self, rhs: &SeqSlice<Iupac>) -> bool {
if rhs.len() != self.len() {
panic!("Cannot compare IUPAC sequences of different length");
}
let slice: &SeqSlice<Iupac> = self;
let intersection: &SeqSlice<Iupac> = &(slice & rhs);
intersection == rhs
}
}
#[macro_export]
macro_rules! iupac {
($seq:expr) => {
match Seq::<Iupac>::from_str($seq) {
Ok(s) => s,
Err(_) => panic!(),
}
};
}