BarcodeConversions

Struct BarcodeConversions 

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pub struct BarcodeConversions {
    pub samples_barcode_hash: HashMap<String, String>,
    pub sample_seqs: AHashSet<String>,
    pub counted_barcodes_hash: Vec<HashMap<String, String>>,
    pub counted_barcode_seqs: Vec<AHashSet<String>>,
}
Expand description

Contains all possible barcode sequences for error handling and barcode to ID conversion

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§samples_barcode_hash: HashMap<String, String>§sample_seqs: AHashSet<String>§counted_barcodes_hash: Vec<HashMap<String, String>>§counted_barcode_seqs: Vec<AHashSet<String>>

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impl BarcodeConversions

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pub fn new() -> Self

Creates an empty BarcodeConversions struct

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pub fn sample_barcode_file_conversion( &mut self, barcode_path: &str, ) -> Result<()>

Reads in comma separated barcode file (CSV). The columns need to have headers. The first column needs to be the nucleotide barcode and the second needs to be the ID

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pub fn barcode_file_conversion( &mut self, barcode_path: &str, barcode_num: usize, ) -> Result<()>

Reads in comma separated barcode file (CSV). The columns need to have headers. The first column needs to be the nucleotide barcode the second needs to be the ID, and the third needs to be the barcode index location

§Panics

This panics if the third column of the barcode conversion file does not contain integers. Also panics if not all integers for barcode numbers is within this columns

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pub fn get_sample_seqs(&mut self)

Creates a hashmap of all sample barcode sequences in order to compare for sequencing errors

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pub fn get_barcode_seqs(&mut self)

Creates a hashmap of all counted barcode sequences in order to compare for sequencing errors

Trait Implementations§

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impl Default for BarcodeConversions

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fn default() -> Self

Returns the “default value” for a type. Read more

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