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use anyhow::Result;
use rust_htslib::{bam, bam::ext::BamRecordExtensions};
use std::fmt::Display;
use std::fs::File;
use std::io::{self, BufWriter, Write};
use std::path::PathBuf;
pub mod cli;
pub mod extract;
pub fn merge_two_lists<T>(left: &[T], right: &[T]) -> Vec<T>
where
T: Ord,
T: Clone,
{
let mut x: Vec<T> = left.iter().chain(right.iter()).cloned().collect();
x.sort();
x
}
pub fn positions_on_complimented_sequence(
record: &bam::Record,
input_positions: &[i64],
) -> Vec<i64> {
let positions: Vec<i64> = if record.is_reverse() {
let seq_len = i64::try_from(record.seq_len()).unwrap();
input_positions.iter().rev().map(|p| seq_len - p).collect()
} else {
input_positions.to_vec()
};
positions
}
pub fn search_sorted<T>(a: &[T], v: &[T]) -> Vec<usize>
where
T: Ord,
T: Display,
{
let mut indexes = Vec::with_capacity(v.len());
let mut a_idx = 0;
for cur_v in v {
while a_idx < a.len() {
if a_idx == 0 && *cur_v <= a[a_idx] {
indexes.push(0);
break;
} else if a_idx == 0 {
a_idx += 1;
}
if a_idx == a.len() - 1 && *cur_v > a[a_idx] {
indexes.push(a_idx + 1);
break;
}
else if (a[a_idx - 1] < *cur_v) && (*cur_v <= a[a_idx]) {
indexes.push(a_idx);
break;
}
a_idx += 1;
}
}
indexes
}
pub fn liftover_closest(record: &bam::Record, query_positions: &[i64]) -> Vec<i64> {
let (q_pos, r_pos): (Vec<i64>, Vec<i64>) = record
.aligned_pairs()
.map(|[q_pos, r_pos]| (q_pos, r_pos))
.unzip();
let ref_idxs = search_sorted(&q_pos, query_positions);
let mut ref_positions = vec![];
for mut idx in ref_idxs {
if idx == r_pos.len() {
idx -= 1;
}
ref_positions.push(r_pos[idx]);
}
ref_positions
}
pub fn liftover_exact(record: &bam::Record, positions: &[i64]) -> Vec<i64> {
let mut ref_positions = vec![];
let mut cur_pos = 0;
for [q_pos, r_pos] in record.aligned_pairs() {
while cur_pos < positions.len() && positions[cur_pos] <= q_pos {
if positions[cur_pos] == q_pos {
ref_positions.push(r_pos);
}
cur_pos += 1;
}
if cur_pos == positions.len() {
break;
}
}
ref_positions
}
pub fn get_closest_reference_positions(positions: &[i64], record: &bam::Record) -> Vec<i64> {
let positions = positions_on_complimented_sequence(record, positions);
log::trace!("seq comp {:?}", positions);
liftover_closest(record, &positions)
}
pub fn get_closest_reference_range(
starts: &[i64],
lengths: &[i64],
record: &bam::Record,
) -> Vec<(i64, i64)> {
let mol_ends: Vec<i64> = starts
.iter()
.zip(lengths.iter())
.map(|(start, length)| start + length)
.collect();
let ref_starts = get_closest_reference_positions(starts, record);
let ref_ends = get_closest_reference_positions(&mol_ends, record);
assert_eq!(ref_starts.len(), ref_ends.len());
ref_starts
.iter()
.zip(ref_ends.iter())
.filter(|(&start, &end)| end - start > 0)
.map(|(&start, &end)| (start, end - start))
.collect()
}
const BUFFER_SIZE: usize = 32 * 1024;
fn get_output(path: Option<PathBuf>) -> Result<Box<dyn Write + Send + 'static>> {
let writer: Box<dyn Write + Send + 'static> = match path {
Some(path) => {
if path.as_os_str() == "-" {
Box::new(BufWriter::with_capacity(BUFFER_SIZE, io::stdout()))
} else {
Box::new(BufWriter::with_capacity(BUFFER_SIZE, File::create(path)?))
}
}
None => Box::new(BufWriter::with_capacity(BUFFER_SIZE, io::stdout())),
};
Ok(writer)
}
pub fn writer(filename: &str) -> Result<Box<dyn Write>> {
let path = PathBuf::from(filename);
let buffer = get_output(Some(path))?;
Ok(buffer)
}
pub struct FiberOutFiles {
pub m6a: Option<Box<dyn Write>>,
pub cpg: Option<Box<dyn Write>>,
pub msp: Option<Box<dyn Write>>,
pub nuc: Option<Box<dyn Write>>,
pub all: Option<Box<dyn Write>>,
}
impl FiberOutFiles {
pub fn new(
m6a: &Option<String>,
cpg: &Option<String>,
msp: &Option<String>,
nuc: &Option<String>,
all: &Option<String>,
) -> Result<Self> {
let m6a = match m6a {
Some(m6a) => Some(writer(m6a)?),
None => None,
};
let cpg = match cpg {
Some(cpg) => Some(writer(cpg)?),
None => None,
};
let msp = match msp {
Some(msp) => Some(writer(msp)?),
None => None,
};
let nuc = match nuc {
Some(nuc) => Some(writer(nuc)?),
None => None,
};
let all = match all {
Some(all) => Some(writer(all)?),
None => None,
};
Ok(FiberOutFiles {
m6a,
cpg,
msp,
nuc,
all,
})
}
}