Crate codonrs

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codonrs is a small crate for rapidly calculating relative synonymous codon usage (RSCU) values for coding DNA sequences, for analyses of codon usage bias. The crate can be used as a command-line utility with the codonrs command, or used in other crates via the analysis mod.

§Command-line usage

§Required arguments

Input: -i/--input: A multi-fasta file with the sequences to be analysed as individual fasta entries. Sequences whose length is not a multiple of three will be ignored

Output: -o/--output: Prefix for the output files. Three files are output by default:

  • prefix_codon.csv: raw codon counts for each CDS.
  • prefix_amino_acids.csv: amino acid counts for each CDS, determined from the chosen translation table.
  • prefix_rscu.csv: calculated RSCU values for each CDS.

§Optional arguments

Translation table: -t/--table: Integer representing NCBI translation table to be used for codon counts and RSCU calculation. See tables here. Defaults to 1: the standard code.

Modules§

analysis