1
  2
  3
  4
  5
  6
  7
  8
  9
 10
 11
 12
 13
 14
 15
 16
 17
 18
 19
 20
 21
 22
 23
 24
 25
 26
 27
 28
 29
 30
 31
 32
 33
 34
 35
 36
 37
 38
 39
 40
 41
 42
 43
 44
 45
 46
 47
 48
 49
 50
 51
 52
 53
 54
 55
 56
 57
 58
 59
 60
 61
 62
 63
 64
 65
 66
 67
 68
 69
 70
 71
 72
 73
 74
 75
 76
 77
 78
 79
 80
 81
 82
 83
 84
 85
 86
 87
 88
 89
 90
 91
 92
 93
 94
 95
 96
 97
 98
 99
100
101
102
103
104
105
106
107
108
109
110
111
112
113
114
115
116
117
118
119
120
121
122
123
124
125
126
127
128
129
130
131
132
133
134
135
136
137
138
139
140
141
142
143
144
145
146
147
148
149
150
151
152
153
154
155
156
157
158
159
160
161
162
163
164
165
166
167
168
169
170
171
172
173
174
175
176
177
178
179
180
181
182
183
184
185
186
187
188
189
190
191
192
193
194
195
196
197
198
199
200
201
202
203
204
205
206
207
208
209
210
211
212
213
214
215
216
217
218
219
220
221
222
223
224
225
226
227
228
229
230
231
232
233
234
235
236
237
238
239
240
241
242
243
244
245
246
247
248
249
250
251
252
253
254
255
256
257
258
259
260
261
262
263
264
265
266
267
268
269
270
271
272
273
274
275
276
277
278
279
280
281
282
283
284
285
286
287
288
289
290
291
292
293
294
295
296
297
298
299
300
301
302
303
304
305
306
307
308
309
310
311
312
313
314
315
316
317
318
319
320
321
322
323
324
325
326
327
328
329
330
331
332
333
334
335
336
337
338
339
340
341
342
343
344
345
346
347
348
349
350
351
352
353
354
355
356
357
358
359
360
361
362
363
364
365
366
367
368
369
370
371
372
373
374
375
376
377
378
379
380
381
382
383
384
385
386
387
388
389
390
391
392
393
394
395
396
397
398
399
400
401
402
403
404
405
406
407
408
409
410
411
412
413
414
415
416
417
418
419
//! CRAM reader and record iterator.

mod container;
pub(crate) mod data_container;
pub(crate) mod num;
pub(crate) mod record;
mod records;

use crate::data_container::DataContainer;

pub use self::records::Records;

use std::{
    io::{self, Read, Seek, SeekFrom},
    str,
};

use byteorder::{LittleEndian, ReadBytesExt};

use self::container::read_container;
use super::{container::Block, file_definition::Version, FileDefinition, MAGIC_NUMBER};

/// A CRAM reader.
///
/// The CRAM format is comprised of four main parts: 1) a file definition, 2) a file header, 3) a
/// list of data containers, and 4) an end-of-file (EOF) container.
///
/// # Examples
///
/// ```no_run
/// # use std::{fs::File, io};
/// use noodles_cram as cram;
///
/// let mut reader = File::open("sample.cram").map(cram::Reader::new)?;
/// reader.read_file_definition()?;
/// reader.read_file_header()?;
///
/// for result in reader.records() {
///     let record = result?;
///     println!("{:?}", record);
/// }
///
/// # Ok::<(), io::Error>(())
/// ```
pub struct Reader<R>
where
    R: Read,
{
    inner: R,
}

impl<R> Reader<R>
where
    R: Read,
{
    /// Creates a CRAM reader.
    ///
    /// # Examples
    ///
    /// ```no_run
    /// # use std::{fs::File, io};
    /// use noodles_cram as cram;
    /// let mut reader = File::open("sample.bam").map(cram::Reader::new)?;
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn new(reader: R) -> Self {
        Self { inner: reader }
    }

    /// Returns a reference to the underlying reader.
    ///
    /// # Examples
    ///
    /// ```
    /// # use std::io;
    /// use noodles_cram as cram;
    /// let data = [];
    /// let reader = cram::Reader::new(&data[..]);
    /// assert!(reader.get_ref().is_empty());
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn get_ref(&self) -> &R {
        &self.inner
    }

    /// Returns a mutable reference to the underlying reader.
    ///
    /// # Examples
    ///
    /// ```
    /// # use std::io;
    /// use noodles_cram as cram;
    /// let data = [];
    /// let mut reader = cram::Reader::new(&data[..]);
    /// assert!(reader.get_mut().is_empty());
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn get_mut(&mut self) -> &mut R {
        &mut self.inner
    }

    /// Unwraps and returns the underlying reader.
    ///
    /// # Examples
    ///
    /// ```
    /// # use std::io;
    /// use noodles_cram as cram;
    /// let data = [];
    /// let reader = cram::Reader::new(&data[..]);
    /// assert!(reader.into_inner().is_empty());
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn into_inner(self) -> R {
        self.inner
    }

    /// Reads the CRAM file definition.
    ///
    /// The CRAM magic number is also checked.
    ///
    /// The position of the stream is expected to be at the start.
    ///
    /// # Examples
    ///
    /// ```no_run
    /// # use std::{fs::File, io};
    /// use noodles_cram as cram;
    /// let mut reader = File::open("sample.cram").map(cram::Reader::new)?;
    /// let file_definition = reader.read_file_definition()?;
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn read_file_definition(&mut self) -> io::Result<FileDefinition> {
        read_magic_number(&mut self.inner)?;

        let format = read_format(&mut self.inner)?;
        let file_id = read_file_id(&mut self.inner)?;

        Ok(FileDefinition::new(format, file_id))
    }

    /// Reads the raw SAM header.
    ///
    /// The position of the stream is expected to be at the CRAM header container, i.e., directly
    /// after the file definition.
    ///
    /// This returns the raw SAM header as a [`String`]. It can subsequently be parsed as a
    /// [`noodles_sam::Header`].
    ///
    /// # Examples
    ///
    /// ```no_run
    /// # use std::{fs::File, io};
    /// use noodles_cram as cram;
    ///
    /// let mut reader = File::open("sample.cram").map(cram::Reader::new)?;
    /// reader.read_file_definition()?;
    ///
    /// let header = reader.read_file_header()?;
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn read_file_header(&mut self) -> io::Result<String> {
        let container = read_container(&mut self.inner)?;

        if let Some(block) = container.blocks().first() {
            read_file_header_block(block)
        } else {
            Err(io::Error::new(
                io::ErrorKind::InvalidData,
                "invalid header container: missing block for SAM header",
            ))
        }
    }

    pub(crate) fn read_data_container_with_container_header(
        &mut self,
    ) -> io::Result<Option<(crate::container::Header, DataContainer)>> {
        use self::data_container::read_data_container_with_container_header;
        read_data_container_with_container_header(&mut self.inner)
    }

    /// Reads a data container.
    ///
    /// This returns `None` if the container header is the EOF container header, which signals the
    /// end of the stream.
    ///
    /// # Examples
    ///
    /// ```no_run
    /// # use std::{fs::File, io};
    /// use noodles_cram as cram;
    ///
    /// let mut reader = File::open("sample.cram").map(cram::Reader::new)?;
    /// reader.read_file_definition()?;
    /// reader.read_file_header()?;
    ///
    /// while let Some(container) = reader.read_data_container()? {
    ///     // ...
    /// }
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn read_data_container(&mut self) -> io::Result<Option<DataContainer>> {
        use self::data_container::read_data_container;

        read_data_container(&mut self.inner)
    }

    /// Returns a iterator over records starting from the current stream position.
    ///
    /// The stream is expected to be at the start of a data container.
    ///
    /// # Examples
    ///
    /// ```no_run
    /// # use std::{fs::File, io};
    /// use noodles_cram as cram;
    ///
    /// let mut reader = File::open("sample.cram").map(cram::Reader::new)?;
    /// reader.read_file_definition()?;
    /// reader.read_file_header()?;
    ///
    /// for result in reader.records() {
    ///     let record = result?;
    ///     println!("{:?}", record);
    /// }
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn records(&mut self) -> Records<'_, R> {
        Records::new(self)
    }
}

impl<R> Reader<R>
where
    R: Read + Seek,
{
    /// Seeks the underlying reader to the given position.
    ///
    /// Positions typically come from the associated CRAM index file.
    ///
    /// # Examples
    ///
    /// ```no_run
    /// # use std::{fs::File, io};
    /// use std::io::SeekFrom;
    /// use noodles_cram as cram;
    ///
    /// let mut reader = File::open("sample.cram").map(cram::Reader::new)?;
    /// reader.seek(SeekFrom::Start(17711))?;
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn seek(&mut self, pos: SeekFrom) -> io::Result<u64> {
        self.inner.seek(pos)
    }

    /// Returns the current position of the underlying reader.
    ///
    /// # Examples
    ///
    /// ```
    /// # use std::io::{self, Cursor};
    /// use noodles_cram as cram;
    /// let data = Cursor::new(Vec::new());
    /// let mut reader = cram::Reader::new(data);
    /// let position = reader.position()?;
    /// assert_eq!(position, 0);
    /// # Ok::<(), io::Error>(())
    /// ```
    pub fn position(&mut self) -> io::Result<u64> {
        self.inner.seek(SeekFrom::Current(0))
    }
}

fn read_magic_number<R>(reader: &mut R) -> io::Result<()>
where
    R: Read,
{
    let mut buf = [0; 4];
    reader.read_exact(&mut buf)?;

    if buf == MAGIC_NUMBER {
        Ok(())
    } else {
        Err(io::Error::new(
            io::ErrorKind::InvalidData,
            "invalid CRAM header",
        ))
    }
}

fn read_format<R>(reader: &mut R) -> io::Result<Version>
where
    R: Read,
{
    let mut buf = [0; 2];
    reader.read_exact(&mut buf)?;
    Ok(Version::new(buf[0], buf[1]))
}

fn read_file_id<R>(reader: &mut R) -> io::Result<[u8; 20]>
where
    R: Read,
{
    let mut buf = [0; 20];
    reader.read_exact(&mut buf)?;
    Ok(buf)
}

pub(crate) fn read_file_header_block(block: &Block) -> io::Result<String> {
    use crate::container::block::ContentType;

    if block.content_type() != ContentType::FileHeader {
        return Err(io::Error::new(
            io::ErrorKind::InvalidData,
            format!(
                "invalid block content type: expected {:?}, got {:?}",
                ContentType::FileHeader,
                block.content_type()
            ),
        ));
    }

    let data = block.decompressed_data()?;
    let mut reader = &data[..];

    let _header_len = reader.read_i32::<LittleEndian>()?;

    str::from_utf8(reader)
        .map(|s| s.into())
        .map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
}

#[cfg(test)]
mod tests {
    use std::convert::TryFrom;

    use crate::container::block::ContentType;

    use super::*;

    #[test]
    fn test_read_file_definition() -> Result<(), Box<dyn std::error::Error>> {
        let data = [
            0x43, 0x52, 0x41, 0x4d, // magic number = b"CRAM"
            0x03, 0x00, // format version = (3, 0)
            0x00, 0x68, 0xac, 0xf3, 0x06, 0x4d, 0xaa, 0x1e, 0x29, 0xa4, 0xa0, 0x8c, 0x56, 0xee,
            0x91, 0x9b, 0x91, 0x04, 0x21, 0x1f, // file ID
        ];

        let mut reader = Reader::new(&data[..]);
        let actual = reader.read_file_definition()?;

        let file_id = <[u8; 20]>::try_from(&data[6..])?;
        let expected = FileDefinition::new(Version::new(3, 0), file_id);

        assert_eq!(actual, expected);

        Ok(())
    }

    #[test]
    fn test_read_file_header_block() -> io::Result<()> {
        let expected = "noodles";

        let header_data = expected.as_bytes();
        let header_data_len = header_data.len() as i32;

        let mut data = header_data_len.to_le_bytes().to_vec();
        data.extend(header_data);

        let block = Block::builder()
            .set_content_type(ContentType::FileHeader)
            .set_uncompressed_len(data.len())
            .set_data(data.to_vec())
            .build();

        let actual = read_file_header_block(&block)?;

        assert_eq!(actual, expected);

        Ok(())
    }

    #[test]
    fn test_read_file_header_block_with_invalid_content_type() {
        let block = Block::builder()
            .set_content_type(ContentType::ExternalData)
            .build();

        assert!(matches!(
            read_file_header_block(&block),
            Err(ref e) if e.kind() == io::ErrorKind::InvalidData
        ));
    }

    #[test]
    fn test_read_magic_number() {
        let data = b"CRAM";
        let mut reader = &data[..];
        assert!(read_magic_number(&mut reader).is_ok());
    }

    #[test]
    fn test_read_magic_number_with_invalid_input() {
        let data = [];
        let mut reader = &data[..];
        assert!(matches!(
            read_magic_number(&mut reader),
            Err(ref e) if e.kind() == io::ErrorKind::UnexpectedEof,
        ));

        let data = b"BAM\x01";
        let mut reader = &data[..];
        assert!(matches!(
            read_magic_number(&mut reader),
            Err(ref e) if e.kind() == io::ErrorKind::InvalidData,
        ));
    }
}