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//! BAM CIGAR and operations.
pub mod op;
mod ops;
pub use self::{op::Op, ops::Ops};
use std::{convert::TryFrom, fmt, io, ops::Deref};
use noodles_sam::{self as sam, record::cigar::op::Kind};
/// BAM record CIGAR.
pub struct Cigar<'a>(&'a [u8]);
impl<'a> Cigar<'a> {
/// Creates a CIGAR by wrapping raw CIGAR data.
///
/// # Examples
///
/// ```
/// use noodles_bam::record::Cigar;
///
/// let data = [
/// 0x40, 0x02, 0x00, 0x00, // 36M
/// 0x84, 0x00, 0x00, 0x00, // 8S
/// ];
///
/// let cigar = Cigar::new(&data);
///
/// assert_eq!(*cigar, data);
/// ```
pub fn new(bytes: &[u8]) -> Cigar<'_> {
Cigar(bytes)
}
/// Returns a iterator over the operations in the CIGAR.
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_bam::record::{cigar::Op, Cigar};
/// use noodles_sam::record::cigar::op::Kind;
///
/// let data = [
/// 0x40, 0x02, 0x00, 0x00, // 36M
/// 0x84, 0x00, 0x00, 0x00, // 8S
/// ];
///
/// let cigar = Cigar::new(&data);
///
/// let mut ops = cigar.ops();
///
/// assert_eq!(ops.next().transpose()?, Some(Op::new(Kind::Match, 36)?));
/// assert_eq!(ops.next().transpose()?, Some(Op::new(Kind::SoftClip, 8)?));
/// assert_eq!(ops.next().transpose()?, None);
/// # Ok::<(), Box<dyn std::error::Error>>(())
/// ```
pub fn ops(&self) -> Ops<'_> {
Ops::new(self.0)
}
/// Calculates the alignment span over the reference sequence.
///
/// This sums the lengths of the CIGAR operations that consume the reference sequence, i.e.,
/// alignment matches (`M`), deletions from the reference (`D`), skipped reference regions
/// (`S`), sequence matches (`=`), and sequence mismatches (`X`).
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_bam::record::{cigar::Op, Cigar};
/// use noodles_sam::record::cigar::op::Kind;
///
/// let data = [
/// 0x40, 0x02, 0x00, 0x00, // 36M
/// 0x43, 0x00, 0x00, 0x00, // 4D
/// 0x84, 0x00, 0x00, 0x00, // 8S
/// ];
///
/// let cigar = Cigar::new(&data);
///
/// assert_eq!(cigar.reference_len()?, 40);
/// # Ok::<(), io::Error>(())
/// ```
pub fn reference_len(&self) -> io::Result<u32> {
let mut len = 0;
for result in self.ops() {
let op = result?;
match op.kind() {
Kind::Match | Kind::Deletion | Kind::Skip | Kind::SeqMatch | Kind::SeqMismatch => {
len += op.len();
}
_ => {}
}
}
Ok(len)
}
/// Calculates the read length.
///
/// This sums the lengths of the CIGAR operations that consume the read, i.e., alignment
/// matches (`M`), insertions to the reference (`I`), soft clips (`S`), sequence matches (`=`),
/// and sequence mismatches (`X`).
///
/// # Examples
///
/// ```
/// # use std::io;
/// use noodles_bam::record::{cigar::Op, Cigar};
/// use noodles_sam::record::cigar::op::Kind;
///
/// let data = [
/// 0x40, 0x02, 0x00, 0x00, // 36M
/// 0x43, 0x00, 0x00, 0x00, // 4D
/// 0x84, 0x00, 0x00, 0x00, // 8S
/// ];
///
/// let cigar = Cigar::new(&data);
///
/// assert_eq!(cigar.read_len()?, 44);
/// # Ok::<(), io::Error>(())
/// ```
pub fn read_len(&self) -> io::Result<u32> {
let mut len = 0;
for result in self.ops() {
let op = result?;
match op.kind() {
Kind::Match
| Kind::Insertion
| Kind::SoftClip
| Kind::SeqMatch
| Kind::SeqMismatch => {
len += op.len();
}
_ => {}
}
}
Ok(len)
}
}
impl<'a> fmt::Debug for Cigar<'a> {
fn fmt(&self, fmt: &mut fmt::Formatter<'_>) -> fmt::Result {
fmt.debug_list().entries(self.ops()).finish()
}
}
impl<'a> fmt::Display for Cigar<'a> {
fn fmt(&self, f: &mut fmt::Formatter<'_>) -> fmt::Result {
for result in self.ops() {
let op = result.map_err(|_| fmt::Error)?;
write!(f, "{}", op)?;
}
Ok(())
}
}
impl<'a> Deref for Cigar<'a> {
type Target = [u8];
fn deref(&self) -> &[u8] {
self.0
}
}
impl<'a> TryFrom<Cigar<'a>> for sam::record::Cigar {
type Error = io::Error;
fn try_from(cigar: Cigar<'_>) -> Result<Self, Self::Error> {
let mut ops = Vec::new();
for result in cigar.ops() {
let op = result?;
ops.push(sam::record::cigar::Op::new(op.kind(), op.len()));
}
Ok(Self::from(ops))
}
}
#[cfg(test)]
mod tests {
use super::*;
#[test]
fn test_from_bytes() -> Result<(), Box<dyn std::error::Error>> {
let bytes = [0x40, 0x02, 0x00, 0x00, 0x62, 0x03, 0x00, 0x00];
let cigar = Cigar::new(&bytes);
let mut ops = cigar.ops();
assert_eq!(ops.next().transpose()?, Some(Op::try_from(0x240)?));
assert_eq!(ops.next().transpose()?, Some(Op::try_from(0x362)?));
assert_eq!(ops.next().transpose()?, None);
Ok(())
}
#[test]
fn test_try_from_cigar_for_sam_record_cigar() -> io::Result<()> {
use sam::record::cigar::{op, Op};
let bytes = [0x40, 0x02, 0x00, 0x00, 0x62, 0x03, 0x00, 0x00];
let cigar = Cigar::new(&bytes);
let actual = sam::record::Cigar::try_from(cigar)?;
let expected = sam::record::Cigar::from(vec![
Op::new(op::Kind::Match, 36),
Op::new(op::Kind::Deletion, 54),
]);
assert_eq!(actual, expected);
Ok(())
}
}