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use std::io::{Write, Error, Result, ErrorKind};
use memchr::memchr;
pub trait Record {
fn seq(&self) -> &[u8];
fn head(&self) -> &[u8];
fn qual(&self) -> &[u8];
fn write<W: Write>(&self, writer: &mut W) -> Result<usize>;
fn validate_dna(&self) -> bool {
self.seq().iter().all(|&x| x == b'A' || x == b'C' || x == b'T' || x == b'G')
}
fn validate_dnan(&self) -> bool {
self.seq().iter().all(|&x| x == b'A' || x == b'C' || x == b'T' || x == b'G' || x == b'N')
}
}
#[derive(Debug)]
pub struct RefRecord<'a> {
head: usize,
seq: usize,
sep: usize,
qual: usize,
data: &'a [u8],
}
#[derive(Debug)]
pub struct OwnedRecord {
pub head: Vec<u8>,
pub seq: Vec<u8>,
pub sep: Option<Vec<u8>>,
pub qual: Vec<u8>,
}
#[derive(Debug)]
pub struct IdxRecord {
head: usize,
seq: usize,
sep: usize,
qual: usize,
pub data: (usize, usize),
}
#[inline]
fn trim_winline(line: &[u8]) -> &[u8] {
if let Some((&b'\r', remaining)) = line.split_last() {
remaining
} else {
line
}
}
impl<'a> Record for RefRecord<'a> {
#[inline]
fn head(&self) -> &[u8] {
trim_winline(&self.data[1 .. self.head])
}
#[inline]
fn seq(&self) -> &[u8] {
trim_winline(&self.data[self.head + 1 .. self.seq])
}
#[inline]
fn qual(&self) -> &[u8] {
trim_winline(&self.data[self.sep + 1 .. self.qual])
}
#[inline]
fn write<W: Write>(&self, writer: &mut W) -> Result<usize> {
writer.write_all(&self.data)?;
Ok(self.data.len())
}
}
impl Record for OwnedRecord {
fn head(&self) -> &[u8] {
&self.head
}
fn seq(&self) -> &[u8] {
&self.seq
}
fn qual(&self) -> &[u8] {
&self.qual
}
fn write<W: Write>(&self, writer: &mut W) -> Result<usize> {
let mut written = 0;
written += writer.write(b"@")?;
written += writer.write(self.head())?;
written += writer.write(b"\n")?;
written += writer.write(self.seq())?;
written += writer.write(b"\n")?;
match self.sep {
Some(ref s) => { written += writer.write(s)? }
None => { written += writer.write(b"+")? }
}
written += writer.write(b"\n")?;
written += writer.write(self.qual())?;
written += writer.write(b"\n")?;
Ok(written)
}
}
pub enum IdxRecordResult {
Incomplete,
EmptyBuffer,
Record(IdxRecord),
}
#[inline]
fn read_header(buffer: &[u8]) -> Result<Option<usize>> {
match buffer.first() {
None => { Ok(None) },
Some(&b'@') => {
Ok(memchr(b'\n', buffer))
},
Some(_) => {
return Err(Error::new(ErrorKind::InvalidData,
"Fastq headers must start with '@'"))
}
}
}
#[inline]
fn read_sep(buffer: &[u8]) -> Result<Option<usize>> {
match buffer.first() {
None => { return Ok(None) },
Some(&b'+') => { Ok(memchr(b'\n', buffer)) },
Some(_) => {
return Err(Error::new(ErrorKind::InvalidData,
"Sequence and quality not separated by +"));
}
}
}
impl<'a> RefRecord<'a> {
pub fn to_owned_record(&self) -> OwnedRecord {
OwnedRecord {
seq: self.seq().to_vec(),
qual: self.qual().to_vec(),
head: self.head().to_vec(),
sep: Some(trim_winline(&self.data[self.seq + 1..self.sep]).to_vec())
}
}
}
impl IdxRecord {
#[inline]
pub fn to_ref_record<'a>(&self, buffer: &'a [u8]) -> RefRecord<'a> {
let data = &buffer[self.data.0..self.data.1];
let datalen = data.len();
debug_assert!(datalen == self.data.1 - self.data.0);
debug_assert!(self.head < datalen);
debug_assert!(self.qual < datalen);
debug_assert!(self.seq < datalen);
debug_assert!(self.sep < datalen);
debug_assert!(self.head < self.seq);
debug_assert!(self.seq < self.sep);
debug_assert!(self.sep < self.qual);
RefRecord {
data: data,
head: self.head,
seq: self.seq,
sep: self.sep,
qual: self.qual,
}
}
#[inline]
pub fn from_buffer(buffer: &[u8]) -> Result<IdxRecordResult> {
if buffer.len() == 0 {
return Ok(IdxRecordResult::EmptyBuffer);
}
let head_end = match read_header(buffer)? {
None => { return Ok(IdxRecordResult::Incomplete) },
Some(val) => val
};
let pos = head_end + 1;
let buffer_ = &buffer[pos..];
let seq_end = match memchr(b'\n', buffer_) {
None => { return Ok(IdxRecordResult::Incomplete) },
Some(end) => end + pos
};
let pos = seq_end + 1;
let buffer_ = &buffer[pos..];
let sep_end = match read_sep(buffer_)? {
None => { return Ok(IdxRecordResult::Incomplete) },
Some(end) => end + pos,
};
let pos = sep_end + 1;
let buffer_ = &buffer[pos..];
let qual_end = match memchr(b'\n', buffer_) {
None => { return Ok(IdxRecordResult::Incomplete) },
Some(end) => end + pos,
};
if qual_end - sep_end != seq_end - head_end {
return Err(Error::new(ErrorKind::InvalidData,
"Sequence and quality length mismatch"));
}
Ok(IdxRecordResult::Record(
IdxRecord {
data: (0, qual_end + 1),
head: head_end,
seq: seq_end,
sep: sep_end,
qual: qual_end,
}
))
}
}