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mod builder;
mod query;
#[allow(dead_code)]
mod record;
pub use self::builder::Builder;
use futures::{stream, Stream};
use noodles_bgzf as bgzf;
use noodles_core::Region;
use noodles_csi::{binning_index::ReferenceSequenceExt, BinningIndex};
use noodles_sam::header::{ReferenceSequence, ReferenceSequences};
use tokio::io::{self, AsyncRead, AsyncReadExt, AsyncSeek};
use self::{query::query, record::read_record};
use crate::{
reader::{bytes_with_nul_to_string, resolve_region},
Record, MAGIC_NUMBER,
};
/// An async BAM reader.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use futures::TryStreamExt;
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::AsyncReader::new)?;
/// reader.read_header().await?;
/// reader.read_reference_sequences().await?;
///
/// let mut records = reader.records();
///
/// while let Some(record) = records.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub struct Reader<R>
where
R: AsyncRead,
{
inner: bgzf::AsyncReader<R>,
}
impl<R> Reader<R>
where
R: AsyncRead + Unpin,
{
/// Creates an async BAM reader builder.
///
/// # Examples
///
/// ```
/// use noodles_bam as bam;
/// let data = [];
/// let builder = bam::AsyncReader::builder(&data[..]);
/// let reader = builder.build();
/// ```
pub fn builder(inner: R) -> Builder<R> {
Builder::new(inner)
}
/// Creates an async BAM reader.
///
/// # Examples
///
/// ```
/// use noodles_bam as bam;
/// let data = [];
/// let reader = bam::AsyncReader::new(&data[..]);
/// ```
pub fn new(reader: R) -> Self {
Self {
inner: bgzf::AsyncReader::new(reader),
}
}
/// Reads the raw SAM header.
///
/// The BAM magic number is also checked.
///
/// The position of the stream is expected to be at the start.
///
/// This returns the raw SAM header as a [`String`]. It can subsequently be parsed as a
/// [`noodles_sam::Header`].
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::AsyncReader::new)?;
/// let header = reader.read_header().await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_header(&mut self) -> io::Result<String> {
read_magic(&mut self.inner).await?;
read_header(&mut self.inner).await
}
/// Reads the binary reference sequences after the SAM header.
///
/// This is not the same as the `@SQ` records in the SAM header. A BAM has a list of reference
/// sequences containing name and length tuples after the SAM header and before the list of
/// records.
///
/// The position of the stream is expected to be directly after the header.
///
/// This returns a reference sequence dictionary ([`noodles_sam::header::ReferenceSequences`]),
/// which can be used to build a minimal [`noodles_sam::Header`] if the SAM header is empty.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::AsyncReader::new)?;
/// reader.read_header().await?;
/// let reference_sequences = reader.read_reference_sequences().await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_reference_sequences(&mut self) -> io::Result<ReferenceSequences> {
read_reference_sequences(&mut self.inner).await
}
/// Reads a single record.
///
/// The record block size (`bs`) is read from the underlying stream, and `bs` bytes are read
/// into the given record buffer.
///
/// The stream is expected to be directly after the reference sequences or at the start of
/// another record.
///
/// It is more ergonomic to read records using a stream (see [`Self::records`] and
/// [`Self::query`]), but using this method directly allows the reuse of a single [`Record`]
/// buffer.
///
/// If successful, the record block size is returned. If a block size of 0 is returned, the
/// stream reached EOF.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::AsyncReader::new)?;
/// reader.read_header().await?;
/// reader.read_reference_sequences().await?;
///
/// let mut record = bam::Record::default();
/// reader.read_record(&mut record).await?;
/// # Ok(())
/// # }
/// ```
pub async fn read_record(&mut self, record: &mut Record) -> io::Result<usize> {
read_record(&mut self.inner, record).await
}
/// Returns an (async) stream over records starting from the current (input) stream position.
///
/// The (input) stream is expected to be directly after the reference sequences or at the start
/// of another record.
///
/// # Examples
///
/// ```no_run
/// # use std::io;
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use futures::TryStreamExt;
/// use noodles_bam as bam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::AsyncReader::new)?;
/// reader.read_header().await?;
/// reader.read_reference_sequences().await?;
///
/// let mut records = reader.records();
///
/// while let Some(record) = records.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub fn records(&mut self) -> impl Stream<Item = io::Result<Record>> + '_ {
Box::pin(stream::try_unfold(
(&mut self.inner, Record::default()),
|(mut reader, mut record)| async {
read_record(&mut reader, &mut record)
.await
.map(|n| match n {
0 => None,
_ => Some((record.clone(), (reader, record))),
})
},
))
}
/// Returns the current virtual position of the underlying BGZF reader.
///
/// # Examples
///
/// ```
/// use noodles_bam as bam;
/// use noodles_bgzf as bgzf;
///
/// let data = Vec::new();
/// let reader = bam::AsyncReader::new(&data[..]);
/// let virtual_position = reader.virtual_position();
///
/// assert_eq!(reader.virtual_position(), bgzf::VirtualPosition::from(0));
/// ```
pub fn virtual_position(&self) -> bgzf::VirtualPosition {
self.inner.virtual_position()
}
}
impl<R> Reader<R>
where
R: AsyncRead + AsyncSeek + Unpin,
{
/// Seeks the underlying BGZF reader to the given virtual position.
///
/// Virtual positions typically come from the associated BAM index file.
///
/// # Examples
///
/// ```
/// # use std::io::{self, Cursor};
/// #
/// # #[tokio::main]
/// # async fn main() -> io::Result<()> {
/// use noodles_bam as bam;
/// use noodles_bgzf as bgzf;
///
/// let data = [];
/// let mut reader = bam::AsyncReader::new(Cursor::new(data));
///
/// let virtual_position = bgzf::VirtualPosition::default();
/// reader.seek(virtual_position).await?;
/// # Ok(())
/// # }
/// ```
pub async fn seek(&mut self, pos: bgzf::VirtualPosition) -> io::Result<bgzf::VirtualPosition> {
self.inner.seek(pos).await
}
/// Returns a stream over records that intersect the given region.
///
/// # Examples
///
/// ```no_run
/// # #[tokio::main]
/// # async fn main() -> Result<(), Box<dyn std::error::Error>>{
/// use futures::TryStreamExt;
/// use noodles_bam::{self as bam, bai};
/// use noodles_core::Region;
/// use noodles_sam as sam;
/// use tokio::fs::File;
///
/// let mut reader = File::open("sample.bam").await.map(bam::AsyncReader::new)?;
/// let header: sam::Header = reader.read_header().await?.parse()?;
///
/// let reference_sequences = header.reference_sequences();
/// let index = bai::r#async::read("sample.bam.bai").await?;
/// let region = Region::mapped("sq0", 8..=13);
/// let mut query = reader.query(reference_sequences, &index, ®ion)?;
///
/// while let Some(record) = query.try_next().await? {
/// // ...
/// }
/// # Ok(())
/// # }
/// ```
pub fn query<I, RS>(
&mut self,
reference_sequences: &ReferenceSequences,
index: &I,
region: &Region,
) -> io::Result<impl Stream<Item = io::Result<Record>> + '_>
where
I: BinningIndex<RS>,
RS: ReferenceSequenceExt,
{
let (reference_sequence_id, interval) = resolve_region(reference_sequences, region)?;
let chunks = index.query(reference_sequence_id, interval)?;
Ok(query(self, chunks, reference_sequence_id, interval))
}
}
async fn read_magic<R>(reader: &mut R) -> io::Result<()>
where
R: AsyncRead + Unpin,
{
let mut magic = [0; 4];
reader.read_exact(&mut magic).await?;
if magic == MAGIC_NUMBER {
Ok(())
} else {
Err(io::Error::new(
io::ErrorKind::InvalidData,
"invalid BAM header",
))
}
}
async fn read_header<R>(reader: &mut R) -> io::Result<String>
where
R: AsyncRead + Unpin,
{
let l_text = reader.read_u32_le().await.and_then(|n| {
usize::try_from(n).map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
})?;
let mut text = vec![0; l_text];
reader.read_exact(&mut text).await?;
// § 4.2 The BAM format (2021-06-03): "Plain header text in SAM; not necessarily
// NUL-terminated".
bytes_with_nul_to_string(&text).or_else(|_| {
String::from_utf8(text).map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
})
}
async fn read_reference_sequences<R>(reader: &mut R) -> io::Result<ReferenceSequences>
where
R: AsyncRead + Unpin,
{
let n_ref = reader.read_u32_le().await.and_then(|n| {
usize::try_from(n).map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
})?;
let mut reference_sequences = ReferenceSequences::with_capacity(n_ref);
for _ in 0..n_ref {
let reference_sequence = read_reference_sequence(reader).await?;
let name = reference_sequence.name().to_string();
reference_sequences.insert(name, reference_sequence);
}
Ok(reference_sequences)
}
async fn read_reference_sequence<R>(reader: &mut R) -> io::Result<ReferenceSequence>
where
R: AsyncRead + Unpin,
{
let l_name = reader.read_u32_le().await.and_then(|n| {
usize::try_from(n).map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
})?;
let mut c_name = vec![0; l_name];
reader.read_exact(&mut c_name).await?;
let name = bytes_with_nul_to_string(&c_name).and_then(|name| {
name.parse()
.map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
})?;
let l_ref = reader.read_u32_le().await.and_then(|len| {
i32::try_from(len).map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
})?;
ReferenceSequence::new(name, l_ref).map_err(|e| io::Error::new(io::ErrorKind::InvalidData, e))
}
#[cfg(test)]
mod tests {
use noodles_sam as sam;
use super::*;
#[tokio::test]
async fn test_read_magic() {
let data = b"BAM\x01";
let mut reader = &data[..];
assert!(read_magic(&mut reader).await.is_ok());
let data = [];
let mut reader = &data[..];
assert!(matches!(
read_magic(&mut reader).await,
Err(ref e) if e.kind() == io::ErrorKind::UnexpectedEof
));
let data = b"MThd";
let mut reader = &data[..];
assert!(matches!(
read_magic(&mut reader).await,
Err(ref e) if e.kind() == io::ErrorKind::InvalidData
));
}
#[tokio::test]
async fn test_read_header() -> io::Result<()> {
let expected = "@HD\tVN:1.6\n";
let data_len = expected.len() as u32;
let mut data = data_len.to_le_bytes().to_vec();
data.extend(expected.as_bytes());
let mut reader = &data[..];
let actual = read_header(&mut reader).await?;
assert_eq!(actual, expected);
Ok(())
}
#[tokio::test]
async fn test_read_reference_sequences() -> Result<(), Box<dyn std::error::Error>> {
use sam::header::reference_sequence;
let data = [
0x01, 0x00, 0x00, 0x00, // n_ref = 1
0x04, 0x00, 0x00, 0x00, // ref[0].l_name = 4
0x73, 0x71, 0x30, 0x00, // ref[0].name = "sq0\x00"
0x08, 0x00, 0x00, 0x00, // ref[0].l_ref = 8
];
let mut reader = &data[..];
let actual = read_reference_sequences(&mut reader).await?;
let expected: ReferenceSequences = [("sq0".parse()?, 8)]
.into_iter()
.map(|(name, len): (reference_sequence::Name, i32)| {
let sn = name.to_string();
ReferenceSequence::new(name, len).map(|rs| (sn, rs))
})
.collect::<Result<_, _>>()?;
assert_eq!(actual, expected);
Ok(())
}
}