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//! Indexed and consecutive BAM readers. use std::fs::File; use std::io::{Read, Seek, Result, Error, Write}; use std::io::ErrorKind::InvalidInput; use std::path::{Path, PathBuf}; use std::result; use super::index::{self, Index}; use super::record; use super::bgzip; use super::header::Header; use super::RecordReader; /// Iterator over records in a specific region. Implements [RecordReader](trait.RecordReader.html) trait. /// /// If possible, create a single record using [Record::new](../record/struct.Record.html#method.new) /// and then use [read_into](trait.RecordReader.html#method.read_into) instead of iterating, /// as it saves time on allocation. pub struct RegionViewer<'a, R: Read + Seek> { chunks_reader: bgzip::ChunksReader<'a, R>, start: i32, end: i32, predicate: Box<Fn(&record::Record) -> bool>, } impl<'a, R: Read + Seek> RecordReader for RegionViewer<'a, R> { fn read_into(&mut self, record: &mut record::Record) -> result::Result<(), record::Error> { loop { if let Err(e) = record.fill_from_bam(&mut self.chunks_reader) { record.clear(); return Err(e); } if !record.flag().is_mapped() { continue; } // Reads are sorted, so no more reads would be in the region. if record.start() >= self.end { record.clear(); return Err(record::Error::NoMoreRecords); } if !(self.predicate)(&record) { continue; } let record_bin = record.calculate_bin(); if record_bin > index::MAX_BIN { record.clear(); return Err(record::Error::Corrupted( "Read has BAI bin bigger than max possible value".to_string())); } let (min_start, max_end) = index::bin_to_region(record_bin); if min_start >= self.start && max_end <= self.end { return Ok(()); } let record_end = record.calculate_end(); if record_end != -1 && record_end < record.start() { record.clear(); return Err(record::Error::Corrupted("aln_end < aln_start".to_string())); } if record_end > self.start { return Ok(()); } } } } /// Iterator over records. /// /// # Errors /// /// If the record was corrupted, the function returns /// [Corrupted](../record/enum.Error.html#variant.Corrupted) error. /// If the record was truncated or the reading failed for a different reason, the function /// returns [Truncated](../record/enum.Error.html#variant.Truncated) error. impl<'a, R: Seek + Read> Iterator for RegionViewer<'a, R> { type Item = result::Result<record::Record, record::Error>; fn next(&mut self) -> Option<Self::Item> { let mut record = record::Record::new(); match self.read_into(&mut record) { Ok(()) => Some(Ok(record)), Err(record::Error::NoMoreRecords) => None, Err(e) => Some(Err(e)), } } } /// Defines how to react to a BAI index being younger than BAM file. /// /// # Variants /// * `Error` - [IndexedReader](struct.IndexedReader.html) will not be constructed if the BAI /// index is was modified earlier than the BAM file. `io::Error` will be raised. /// * `Ignore` - does nothing if the index is younger than the BAM file. /// * `Warn` - calls a function `Fn(&str)` and continues constructing /// [IndexedReader](struct.IndexedReader.html); pub enum ModificationTime { Error, Ignore, Warn(Box<Fn(&str)>), } impl ModificationTime { fn check<T: AsRef<Path>, U: AsRef<Path>>(&self, bam_path: T, bai_path: U) -> Result<()> { let bam_modified = bam_path.as_ref().metadata().and_then(|metadata| metadata.modified()); let bai_modified = bai_path.as_ref().metadata().and_then(|metadata| metadata.modified()); let bam_younger = match (bam_modified, bai_modified) { (Ok(bam_time), Ok(bai_time)) => bai_time < bam_time, _ => false, // Modification time not available. }; if !bam_younger { return Ok(()); } match &self { ModificationTime::Ignore => {}, ModificationTime::Error => return Err(Error::new(InvalidInput, "the BAM file is younger than the BAI index")), ModificationTime::Warn(box_fun) => box_fun("the BAM file is younger than the BAI index"), } Ok(()) } /// Create a warning strategy `ModificationTime::Warn`. pub fn warn<F: Fn(&str) + 'static>(warning: F) -> Self { ModificationTime::Warn(Box::new(warning)) } } /// [IndexedReader](struct.IndexedReader.html) builder. Allows to specify paths to BAM and BAI /// files, as well as LRU cache size and an option to ignore or warn BAI modification time check. pub struct IndexedReaderBuilder { cache_capacity: Option<usize>, bai_path: Option<PathBuf>, modification_time: ModificationTime, } impl IndexedReaderBuilder { /// Creates a new indexed reader builder. pub fn new() -> Self { Self { cache_capacity: None, bai_path: None, modification_time: ModificationTime::Error, } } /// Sets a path to a BAI index. By default, it is `{bam_path}.bai`. /// Overwrites the last value, if any. pub fn bai_path<P: AsRef<Path>>(&mut self, path: P) -> &mut Self { self.bai_path = Some(path.as_ref().to_path_buf()); self } /// By default, [IndexedReader::new](struct.IndexedReader.html#method.new) and /// [IndexedReaderBuilder::from_path](struct.IndexedReaderBuilder.html#method.from_path) /// return an `io::Error` if the modification time of the BAI index is earlier /// than the modification time of the BAM file. /// /// Enum [ModificationTime](enum.ModificationTime.html) contains options to skip /// this check or raise a warning instead of returning an error. pub fn modification_time(&mut self, modification_time: ModificationTime) -> &mut Self { self.modification_time = modification_time; self } /// Sets new LRU cache capacity. See /// [cache_capacity](../bgzip/struct.SeekReaderBuilder.html#method.cache_capacity) /// for more details. pub fn cache_capacity(&mut self, cache_capacity: usize) -> &mut Self { assert!(cache_capacity > 0, "Cache size must be non-zero"); self.cache_capacity = Some(cache_capacity); self } /// Creates a new [IndexedReader](struct.IndexedReader.html) from `bam_path`. /// If BAI path was not specified, the functions tries to open `{bam_path}.bai`. pub fn from_path<P: AsRef<Path>>(&self, bam_path: P) -> Result<IndexedReader<File>> { let bam_path = bam_path.as_ref(); let bai_path = self.bai_path.as_ref().map(PathBuf::clone) .unwrap_or_else(|| PathBuf::from(format!("{}.bai", bam_path.display()))); self.modification_time.check(&bam_path, &bai_path)?; let mut reader_builder = bgzip::SeekReader::build(); if let Some(cache_capacity) = self.cache_capacity { reader_builder.cache_capacity(cache_capacity); } let reader = reader_builder.from_path(bam_path) .map_err(|e| Error::new(e.kind(), format!("Failed to open BAM file: {}", e)))?; let index = Index::from_path(bai_path) .map_err(|e| Error::new(e.kind(), format!("Failed to open BAI index: {}", e)))?; IndexedReader::new(reader, index) } /// Creates a new [IndexedReader](struct.IndexedReader.html) from two streams. /// BAM stream should support random access, while BAI stream does not need to. /// `check_time` and `bai_path` values are ignored. pub fn from_streams<R: Seek + Read, T: Read>(&self, bam_stream: R, bai_stream: T) -> Result<IndexedReader<R>> { let mut reader_builder = bgzip::SeekReader::build(); if let Some(cache_capacity) = self.cache_capacity { reader_builder.cache_capacity(cache_capacity); } let reader = reader_builder.from_stream(bam_stream); let index = Index::from_stream(bai_stream) .map_err(|e| Error::new(e.kind(), format!("Failed to open BAI index: {}", e)))?; IndexedReader::new(reader, index) } } /// BAM file reader. In contrast to [BamReader](struct.BamReader.html) the `IndexedReader` /// allows to fetch records from arbitrary positions, /// but does not allow to read all records consecutively. /// /// The following code would load BAM file `in.bam` and its index `in.bam.bai`, take all records /// from `3:600001-700000` and print them on the stdout. /// /// ```rust /// extern crate bam; /// /// fn main() { /// let mut reader = bam::IndexedReader::from_path("in.bam").unwrap(); /// /// // We need to clone the header to have access to reference names as the /// // reader will be blocked during fetch. /// let header = reader.header().clone(); /// let mut stdout = std::io::BufWriter::new(std::io::stdout()); /// /// for record in reader.fetch(2, 600_000, 700_000).unwrap() { /// record.unwrap().write_sam(&mut stdout, &header).unwrap(); /// } /// } /// ``` /// /// Additionally, you can use `read_into(&mut record)` to save time on record allocation: /// ```rust /// extern crate bam; /// /// // You need to import RecordReader trait /// use bam::RecordReader; /// /// fn main() { /// let mut reader = bam::IndexedReader::from_path("in.bam").unwrap(); /// /// let header = reader.header().clone(); /// let mut stdout = std::io::BufWriter::new(std::io::stdout()); /// /// let mut viewer = reader.fetch(1, 100_000, 200_000).unwrap(); /// let mut record = bam::Record::new(); /// loop { /// match viewer.read_into(&mut record) { /// Ok(()) => {}, /// Err(bam::Error::NoMoreRecords) => break, /// Err(e) => panic!("{}", e), /// } /// record.write_sam(&mut stdout, &header).unwrap(); /// } /// } /// ``` /// /// If only records with specific MAPQ or FLAGs are needed, you can use `fetch_by`. For example, /// ```rust /// reader.fetch_by(1, 100_000, 200_000, /// |record| record.mapq() >= 30 && !record.flag().is_secondary()) /// ``` /// to load only records with MAPQ at least 30 and skip all secondary alignments. In some cases it /// helps to save time by not calculating the right-most aligned read position, as well as /// skip unnecessary allocations. /// /// You can also use [IndexedReaderBuilder](struct.IndexedReaderBuilder.html), /// which gives more control over loading /// [IndexedReader](struct.IndexedReader.html). /// For example you can create a reader using a different BAI path, and a different cache capacity: /// ```rust /// let mut reader = bam::IndexedReader::build() /// .bai_path("other_dir/test.bai") /// .cache_capacity(10000) /// .from_path("in.bam").unwrap(); /// ``` /// /// By default, during the construction of the `IndexedReader`, we compare modification times of /// the BAI index and the BAM file. If the index is older, the function returns an error. This can /// be changed: /// ```rust /// use bam::bam_reader::ModificationTime; /// let mut reader = bam::IndexedReader::build() /// .modification_time(ModificationTime::warn(|e| eprintln!("{}", e))) /// .from_path("in.bam").unwrap(); /// ``` /// You can also ignore the error completely: `.modification_time(ModificationTime::Ignore)`. pub struct IndexedReader<R: Read + Seek> { reader: bgzip::SeekReader<R>, header: Header, index: Index, buffer: Vec<u8>, } impl IndexedReader<File> { /// Creates [IndexedReaderBuilder](struct.IndexedReaderBuilder.html). pub fn build() -> IndexedReaderBuilder { IndexedReaderBuilder::new() } /// Opens bam file from `path`. Bai index will be loaded from `{path}.bai`. /// /// Same as `Self::build().from_path(path)`. pub fn from_path<P: AsRef<Path>>(path: P) -> Result<Self> { Self::build().from_path(path) } } impl<R: Read + Seek> IndexedReader<R> { fn new(mut reader: bgzip::SeekReader<R>, index: Index) -> Result<Self> { let mut buffer = Vec::with_capacity(bgzip::MAX_BLOCK_SIZE); let header = { let mut header_reader = bgzip::ChunksReader::without_boundaries( &mut reader, &mut buffer); Header::from_bam(&mut header_reader)? }; Ok(Self { reader, header, index, buffer, }) } /// Returns an iterator over records aligned to the reference `ref_id` (0-based), /// and intersecting half-open interval `[start-end)`. pub fn fetch<'a>(&'a mut self, ref_id: u32, start: u32, end: u32) -> Result<RegionViewer<'a, R>> { self.fetch_by(ref_id, start, end, |_| true) } /// Returns an iterator over records aligned to the reference `ref_id` (0-based), /// and intersecting half-open interval `[start-end)`. /// /// Records will be filtered by `predicate`. It helps to slightly reduce fetching time, /// as some records will be removed without allocating new memory and without calculating /// alignment length. pub fn fetch_by<'a, F>(&'a mut self, ref_id: u32, start: u32, end: u32, predicate: F) -> Result<RegionViewer<'a, R>> where F: 'static + Fn(&record::Record) -> bool { if start > end { return Err(Error::new(InvalidInput, format!("Failed to fetch records: start > end ({} > {})", start, end))); } match self.header.reference_len(ref_id as usize) { None => return Err(Error::new(InvalidInput, format!("Failed to fetch records: out of bounds reference {}", ref_id))), Some(len) if len < end => return Err(Error::new(InvalidInput, format!("Failed to fetch records: end > reference length ({} > {})", end, len))), _ => {}, } let chunks = self.index.fetch_chunks(ref_id, start as i32, end as i32); Ok(RegionViewer { chunks_reader: bgzip::ChunksReader::new(&mut self.reader, chunks, &mut self.buffer), start: start as i32, end: end as i32, predicate: Box::new(predicate), }) } /// Returns [header](../header/struct.Header.html). pub fn header(&self) -> &Header { &self.header } /// Writes record in sam format. /// Same as [Record::write_sam](../record/struct.Record.html#method.write_sam). pub fn write_record_as_sam<W: Write>(&self, writer: &mut W, record: &record::Record) -> Result<()> { record.write_sam(writer, self.header()) } } /// BAM file reader. In contrast to [IndexedReader](struct.IndexedReader.html) the `BamReader` /// allows to read all records consecutively, but does not allow random access. /// /// You can iterate over records: /// ```rust /// extern crate bam; /// /// fn main() { /// let reader = bam::BamReader::from_path("in.bam").unwrap(); /// for record in reader { /// let record = record.unwrap(); /// // Do something. /// } /// } /// ``` /// /// Alternatively, you can skip excessive record allocation using `read_into`: /// ```rust /// extern crate bam; /// /// // You need to import RecordReader trait /// use bam::RecordReader; /// /// fn main() { /// let mut reader = bam::BamReader::from_path("in.bam").unwrap(); /// let mut record = bam::Record::new(); /// loop { /// match reader.read_into(&mut record) { /// Ok(()) => {}, /// Err(bam::Error::NoMoreRecords) => break, /// Err(e) => panic!("{}", e), /// } /// // Do something. /// } /// } /// ``` pub struct BamReader<R: Read> { bgzip_reader: bgzip::ConsecutiveReader<R>, header: Header, } impl BamReader<File> { /// Creates BAM file reader from `path`. pub fn from_path<P: AsRef<Path>>(path: P) -> Result<Self> { let stream = File::open(path) .map_err(|e| Error::new(e.kind(), format!("Failed to open BAM file: {}", e)))?; Self::from_stream(stream) } } impl<R: Read> BamReader<R> { /// Creates BAM file reader from `stream`. The stream does not have to support random access. pub fn from_stream(stream: R) -> Result<Self> { let mut bgzip_reader = bgzip::ConsecutiveReader::from_stream(stream)?; let header = Header::from_bam(&mut bgzip_reader)?; Ok(Self { bgzip_reader, header, }) } /// Returns [header](../header/struct.Header.html) pub fn header(&self) -> &Header { &self.header } } impl<R: Read> RecordReader for BamReader<R> { fn read_into(&mut self, record: &mut record::Record) -> result::Result<(), record::Error> { if let Err(e) = record.fill_from_bam(&mut self.bgzip_reader) { record.clear(); Err(e) } else { Ok(()) } } } /// Iterator over records. /// /// # Errors /// /// If the record was corrupted, the function returns /// [Corrupted](../record/enum.Error.html#variant.Corrupted) error. /// If the record was truncated or the reading failed for a different reason, the function /// returns [Truncated](../record/enum.Error.html#variant.Truncated) error. impl<R: Read> Iterator for BamReader<R> { type Item = result::Result<record::Record, record::Error>; fn next(&mut self) -> Option<Self::Item> { let mut record = record::Record::new(); match self.read_into(&mut record) { Ok(()) => Some(Ok(record)), Err(record::Error::NoMoreRecords) => None, Err(e) => Some(Err(e)), } } }